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宋艳, 张茂林, 关振宏, 段铭, 张菁. miR-29c真核表达载体构建及鉴定[J]. 中国公共卫生, 2012, 28(9): 1241-1243. DOI: 10.11847/zgggws2012-28-09-38
引用本文: 宋艳, 张茂林, 关振宏, 段铭, 张菁. miR-29c真核表达载体构建及鉴定[J]. 中国公共卫生, 2012, 28(9): 1241-1243. DOI: 10.11847/zgggws2012-28-09-38
SONG Yan, ZHANG Mao-lin, GUAN Zhen-hong, . Construction and identification of eukaryotic expression vector of MiR-29c[J]. Chinese Journal of Public Health, 2012, 28(9): 1241-1243. DOI: 10.11847/zgggws2012-28-09-38
Citation: SONG Yan, ZHANG Mao-lin, GUAN Zhen-hong, . Construction and identification of eukaryotic expression vector of MiR-29c[J]. Chinese Journal of Public Health, 2012, 28(9): 1241-1243. DOI: 10.11847/zgggws2012-28-09-38

miR-29c真核表达载体构建及鉴定

Construction and identification of eukaryotic expression vector of MiR-29c

  • 摘要: 目的 构建pcDNA6.2-GW/EmGFP-miR-29c真核表达载体,为进一步研究miR-29c的功能和靶基因鉴定奠定基础。方法 根据miR-29c成熟体序列设计合成1对miRNA寡聚单链DNA,经退火后克隆到真核表达载体pcDNA6.2-GW/EmGFP-miR中,瞬时转染肺腺癌A549细胞后,经实时荧光定量PCR检测pcDNA6.2-GW/EmGFP-miR-29c在肺癌细胞中表达miR-29c。结果 A549转染pcDNA6.2-GW/EmGFP-miR-29c 24 h后,miR-29c表达量上升,明显高于阴性对照,差异有统计学意义(P<0.05)。结论 成功构建真核表达载体pcDNA6.2-GW/EmGFP-miR-29c,并能有效表达miR-29c。

     

    Abstract: Objective To evaluate the effect of the intervention on knowledge of nutrition and health in puerperium among late-pregnant women.Methods Totally 640 late-pregnant women taking antepartum examination in No.8 Qingdao People's Hospital were randomly selected and divided into intervention group and control group.The women of intervention group accepted an education on nutrition and health knowledge.The two groups were followed up.Results Eventually 420 women completed the follow-up (213 in the intervention group,207 in the control group).The aw areness rate of nutritional and health know ledge of the intervention group was significantly higher than that in the control group.Women in the intervention group had more intakes of potato (105.87±68.85 g),fish (153.52±76.47 g),soy products (125.35 ±72.62 g),green leafy vegetables(168.78 ±100.81 g),nuts(40.14 ±41.73 g),dietary fiber(14.11 ±5.14 g),niacin(19.07±6.3 mg),calcium(782.57±294.87 mg),and vitamin C (131.48±76.00 mg) and had a higher score of health behavior(13.03±3.54) than those in control group.Women in the intervention group had less intake of egg (111.34±35.54 g),cholesterol(1494.70±650.18 g) than those in control group(t=1.99,4.30,P< 0.05 or P< 0.01),and had lower rate of disease(51.2%) than that in the control group(χ 2=21.12,P< 0.01).Conclusion The results show that specific nutritional health education could improve the know ledge level of puerperal women and promote healthy dietary and life behavior and reduce puerperal disorders.

     

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