Abstract: Objective To investigate genotype of human papillomavirus(HPV) among female outpatients in major cities in Jiangsu province and to provide fundamental evidence for prevention and treatment of cervical cancer and the study on specific HPV vaccine development.Methods Using gene chip technology, 23 HPV subtypes(18 high-risk types and 5 low-risk types) in cervical exfoliated cells from 4 590 female outpatients in 9 cities including Nanjing, Suzhou, Changzhou, and Yangzhou were detected between July 2011 and March 2012.The single and combination subtype HPV prevalence were analyzed and that of different region and age groups were compared.Results Of the outpatients, 1 777(38.71%) were infected with one or more HPV subtypes.A significant difference in the overall prevalence rate was found among different regions(P<0.001)and age groups(P<0.001).The overall prevalence was higher among the outpatients from Suzhou(45.37%), Wuxi(34.54%) and Changzhou city(40.15%) than other regions.The prevalence rate was the highest in the outpatients of ≤25 years old(43.30%), followed by the rates in outpatients of ≥40 years(42.08%), 31-39 years (36.31%), and the 26-30 years (34.70%).The detection rate of HPV6, HPV11, HPV31, HPV33, HPV59, and HPV73 was significantly different among the outpatients from different regions(P<0.05)and the prevalence for the HPV6, HPV11, HPV43, HPV31, HPV33, HPV51, HPV58, HPV59, and HPV66 was significantly different among the outpatients of different age groups(P<0.05).Conclusion There are significant differences in regional and age distribution for the single and combination subtypes of HPV among the female outpatients in major cities of Jiangsu province.