氯化阻燃剂得克隆对大鼠环境激素作用

孙莹; 王晓溪; 李百祥; 吴艳萍

doi:10.11847/zgggws2014-30-09-17

氯化阻燃剂得克隆对大鼠环境激素作用

doi: 10.11847/zgggws2014-30-09-17

哈尔滨医科大学公共卫生学院卫生毒理学教研究室, 黑龙江哈尔滨 150081

基金项目:

中央高校基本科学研究业务费专项资金（HIT.IBRSEM.2022003）

详细信息

作者简介:
孙莹（1989- ），女，黑龙江佳木斯人，硕士在读，研究方向：环境毒物的类激素作用。

通讯作者:
李百祥，libaix@ems.hrbmu.edu.cn

中图分类号: R994.6
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出版历程
- 收稿日期: 2014-04-04
- 刊出日期: 2014-09-10

Environmental hormone effect of chlorinated flame retardant decholrane plus：a preliminary study

Department of Hygienic Toxicology, Scholl of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, China

摘要

摘要: 目的探讨阻燃剂得克隆的环境激素作用。方法将去势 Wistar 雌鼠随机分为6组，去卵巢（模型）组、假手术组、得克隆低、中、高剂量组（25、50、100 mg/kg），阳性对照组（雌二醇50 mg/kg）；连续灌胃14 d，第15 d处死，收集血清、摘取子宫称重并冷冻保存，通过电化学发光免疫法检测大鼠血清甲状腺激素（FT₃、FT₄、TSH）含量，采用 Real-time RT PCR 法检测子宫雌激素受体 α（Erα）mRNA 表达水平。结果与假手术组比较，模型组大鼠子宫重量[（0.112±0.035）g]与子宫/体重比值（0.072±0.030）明显降低，子宫内 Erα mRNA 表达水平明显下降（P<0.05）；与模型组比较，高剂量得克隆组大鼠子宫重量[（0.419±0.059）g]与子宫/体重比值（0.186±0.143）明显上升，子宫内 Erα mRNA 表达量明显增加，差异有统计学意义（P<0.05）；各组血清甲状腺激素水平无明显变化。结论得克隆在体内具有雌激素样作用，但对血清甲状腺激素无影响。
- 得克隆 /
- 环境激素 /
- 雌激素
Abstract: Objective To examine environmental hormone effects of flame retardant decholrane plus(DP).Methods The ovariectomized(OVX) female Wistar rats were randomly divided into six groups:OVX group,DP treatment groups(25,50,100 mg/kg),positive control group(estradiol[E₂],50 mg/kg),and sham-operated group.The rats were treated once a day for 14 consecutive days and sacrificed on day 15;then,the blood samples were collected and uterus were weighed and stored under frozen.Serum-free triiodothyronine(FT₃),serum-free thyroxine(FT₄),and thyroid stimulating hormone(TSH) levels were measured with electrochemiluminescence immunoassay and uterine estrogen receptor α(Erα) mRNA expression was determined with real-time reverse transcription PCR(RT-PCR).Results Compared with the sham group,the uterine weight(0.112±0.035 g) and uterine/body weight ratio(0.072 ± 0.030) of the model group were significantly reduced and the expression of Erα mRNA level was significantly decreased(P<0.05).Compared with the model group,the uterine weight(0.419±0.059 g) and uterine/body weight ratio(0.186±0.143) of the high-dose DP group increased significantly and the expression of Erα mRNA in the uterus significantly increased,with significant differences(P<0.05).No statisticallly significant changes in serum FT₃,PFT₄,and TSH were observed in the rats with DP treatment.Conclusion DP has estrogenic effect but had no effect on serum thyroid hormone level in rats.
- dechlorane plus /
- environmental hormone /
- estrogen

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参考文献(14)

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