摘要:
目的 观察黄精多糖对力竭小鼠脑组织自由基代谢的影响，探讨其抗疲劳的机制。方法 昆明种小鼠72只，随机分为对照组和黄精多糖低、高剂量组。经口灌胃4周，将3组小鼠分别于安静时、力竭即刻、力竭恢复24 h 3种状态下断头处死，测定脑组织中丙二醛（MDA）含量，超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、总抗氧化能力（T-AOC）的活性。结果 力竭即刻及力竭恢复24 h，高剂量组MDA含量分别为（3.46±0.29）、（2.29±0.55）nmol/mg，明显低于对照组和低剂量组（P<0.01）；高剂量组SOD活性分别为（100.64±10.12）、（114.58±9.50）U/mg，GSH-Px活性分别为（2.17±0.35）、（2.45±0.27）U/mg，与对照组相比差异有统计学意义（P<0.01）；T-AOC活性分别为（0.50±0.09）、（0.75±0.09）U/mg，明显高于对照组和低剂量组（P<0.01）。结论 黄精多糖通过增强小鼠脑组织抗氧化能力，发挥抗疲劳作用。
关键词:
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黄精多糖 /
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脑组织 /
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自由基 /
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抗氧化
Abstract:
Objective To observe the effects of polygonatum sibiricum polysaccharides (PSP) on free radical metabolism in brain tissue of the mice at the time of exhaustion and recovery.Methods Seventy-two Kunming mice were randomly divided into three groups:normal group(N),high (H) and low(L) dose PSP group.The mice in the H and L dose groups were administered with PSP by gavage once a day for 4 weeks and those in the N group were treated with a same amount of normal saline.By the end of the treatment,the mice of the 3 groups were sacrificed at the time of rest state,the immediate end of exhaustive exercise and 24 hours after the end of exhaustive exercise,respectively.The content of malonaldehyde(MDA),the activity of superoxide dismutase (SOD) and glutathione peroxidase(GSH-Px),and total antioxidant capacity(T-AOC)in brain tissue of the mice were measured.Results For the mice in H group,the contents of MDA (3.46±0.29 at the immediate end of exhaustive exercise,2.29±0.55 nmol/mg 24 hours after the end of exhaustive exercise)were significantly lower than those of the mice in N and L group(P<0.01,P<0.05),and the activities of SOD(100.64±10.12,114.58±9.50 U/mg),GSH-Px(2.17±0.35,2.45±0.27 U/mg),and T-AOC(0.50±0.09,0.75±0.09 U/mg)were all higher than those of the mice in N and L group.Conclusion PSP can improve antioxidant capacity of brain tissue and has significant anti-fatigue function in mice.
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