MK-801对大鼠脑部多巴胺含量及Fos蛋白表达影响

吴敏范; 郭丽; 刘忠; 杨宇; 商丽宏; 李娜然; 马积昊

doi:10.11847/zgggws2014-30-09-19

MK-801对大鼠脑部多巴胺含量及Fos蛋白表达影响

doi: 10.11847/zgggws2014-30-09-19

1.
沈阳医学院生理学教研室, 辽宁沈阳 110034;
2.
沈阳医学院科学实验中心;
3.
沈阳医学院形态学实验中心;
4.
辽宁何氏医学院2011级大学生

基金项目:

教育部留学回国人员科研启动基金（教外司留[2008]101号），辽宁省教育厅基金（L2012376;2009A698）;沈阳市科学技术局计划项目（1063317-1-00;F10-205-1-42;F13-301-1-00）

详细信息

作者简介:
吴敏范（1963- ），女，满族，辽宁人，教授，博士，主要从事中枢痛觉感受与镇痛机制研究工作。

中图分类号: R338;Q432
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出版历程
- 收稿日期: 2013-12-02
- 刊出日期: 2014-09-10

Effects of MK-801 on dopamine content and Fos protein expression in rat brain

1.
Department of Physiology, Shenyang Medical College, Shenyang, Liaoning Province 110034, China

摘要

摘要: 目的探讨5-甲基二氢丙环庚烯亚胺马来酸（MK-801）对伤害性电刺激隐神经引起的扣带回前部（ACC）多巴胺含量与Fos蛋白表达影响；N-甲基-D-天冬氨酸（NMDA）受体可能参与隐神经伤害性信息传入引起的ACC 多巴胺含量升高与Fos蛋白表达增强过程。方法 72只雄性Wistar大鼠，随机分为对照组、假刺激组、电刺激组、MK-801低、高剂量组及生理盐水对照组；将大鼠断头、取大脑右侧ACC，匀浆，4 ℃、10 000 r/min离心20 min，取上清液20 μL，应用高效液相色谱-电化学检测技术检测ACC内多巴胺含量；将ACC脑组织连续冠状冰冻切片，用免疫组化技术测定Fos蛋白表达。结果与假刺激组比较，电刺激组大鼠ACC 多巴胺含量[（24.89±2.50）ng/g]、Fos蛋白表达量（8.481 1±1.243 2）和阳性细胞百分比[（3.133 8±0.824 2）%]明显升高；与电刺激组比较，0.5、1.0 mg/kg MK-801组大鼠ACC多巴胺含量[分别为（15.07±3.75）、（14.84±2.92）ng/g]、Fos蛋白表达量[分别为（7.107 8±0.610 7）、（7.037 5±0.411 8）]与阳性细胞百分比[分别为（2.173 9±0.466 8）、（2.047 6±0.361 3）%]明显下降；提示MK-801对伤害性电刺激隐神经引起的大鼠脑ACC多巴胺含量升高与Fos蛋白表达增强具有拮抗作用。结论 NMDA受体可能参与隐神经伤害性信息传入引起的ACC多巴胺含量升高与Fos蛋白表达增强过程。
- 多巴胺 /
- Fos蛋白 /
- N-甲基-D-天冬氨酸(NMDA)受体 /
- 隐神经 /
- 扣带回前部(ACC) /
- 躯体痛
Abstract: Objective To investigate the effects of (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate(MK-801)on the changes of dopamine(DA)content and Fos protein expression in anterior cingulate cortex(ACC)of rats induced by noxious electrical stimulation of saphenous nerve(SN).Methods Seventy-two male Wistar rats were randomly divided into control group,sham stimulation group,electrical stimulation group,low-(0.5 mg/kg body weight)and high-dose(1.0 mg/kg body weight)MK-801 group,and NS control group.The rats were sacrificed after the treatments and then the right side ACC were collected and homogenized.After spinning at 10 000 rpm at 4 ℃ for 20 min,20 μL of the supernatant was harvested from each sample.High performance liquid chromatography electrochemical detection was used to measure DA content in ACC.ACC brain tissues of the different groups were cut to continuous coronal frozen sections.Then immunohistochemistry method was used to measure the Fos protein expression of ACC.Results Compared with those of the sham stimulation group,DA content(24.89±2.50 ng/g),Fos protein expression(8.481 1±1.243 2),and the percentage of positive cells(3.133 8±0.824 2%) in the rats ACC of electrical stimulation group were significantly increased.Compared with those of the electrical stimulation group,DA content(15.07±3.75 and 14.84±2.92 ng/g),Fos protein expression (7.107 8±0.610 7 and 7.037 5±0.411 8),and the percentage of positive cells(2.173 9±0.4668%and 2.047 6±0.361 3%)in the rats ACC of low- and high-dose MK-801 group were significantly decreased,indicating that MK-801 antagonized the increases in DA content and Fos protein expression in ACC caused by noxious electrical stimulation of SN.Conclusion The results suggest that N-methyl-D-aspartate receptor may be involved in the increases in DA content and Fos protein expression of rats ACC induced by nociceptive afferent singnal from SN.
- dopamine /
- Fos protein /
- N-methyl-D-aspartate receptor /
- saphenous nerve /
- anterior cingulate cortex /
- somatic pain

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参考文献(13)

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