基因家庭Pax7与Ubc9基因编码蛋白相互作用研究

刘乙蒙; 栾治东; 刘超; 李明洋; 肖建英

doi:10.11847/zgggws2014-30-09-21

基因家庭Pax7与Ubc9基因编码蛋白相互作用研究

doi: 10.11847/zgggws2014-30-09-21

1.
辽宁医学院基础医学院生物化学与分子生物学教研室, 辽宁锦州 121001;
2.
辽宁医学院基础医学院发育生物学教研室

基金项目:

辽宁省教育厅一般项目资助（L2012303）

详细信息

作者简介:
刘乙蒙（1988- ），女，沈阳人，硕士在读，研究方向：胚胎发育的分子机制。

通讯作者:
肖建英，lyxiaojy@163.com

中图分类号: R293
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出版历程
- 收稿日期: 2014-04-03
- 刊出日期: 2014-09-10

Interaction between gene family Pax7 and gene coding protein Ubc9

1.
Department of Biochemical and Molecular Biology, Liaoning Medical College, Jinzhou, Liaoning Province 121001, China

摘要

摘要: 目的了解Pax7的重要生物学功能，筛选并验证与其相互作用的Ubc9基因编码蛋白，并明确Pax7与小泛素相关修饰蛋白（SUMO）的关系，为进一步研究Pax7的作用机制奠定基础。方法酵母双杂交系统筛选出与Pax7相互作用的基因Ubc9；GST-pull down方法验证Pax7与Ubc9在体外相互作用；Western blot方法检测Pax7的SUMO化状态；免疫共沉淀方法验证Pax7与SUMO的关系。结果 Pax7和Ubc9在酵母中存在相互作用；Pax7和Ubc9在体外可特异性结合；在Ubc9存在的条件下，Pax7呈现SUMO化状态，且可与SUMO相互作用；Pax7在鸡胚胎中也呈现SUMO化状态。结论 Pax7可以与Ubc9相互作用，且这种作用与SUMO有关。
- Pax7 /
- Ubc9 /
- 小泛素相关修饰蛋白(SUMO)
Abstract: Objective To explore biological function of gene family Pax7,its interaction with gene coding protein Ubc9,and the relationship between Pax7 and small ubiquitin-related modifier(SUMO).Methods Yeast two-hybrid was applied to screen out Ubc9 interacted with Pax7.The interaction between Pax7 and Ubc9 in vitro was confirmed with glutathione-S-transferase(GST) pull-down.Western blot was used to detect the SUMOylation of Pax7.The interaction between Pax7 and SUMO was demonstrated with co-immunoprecipitation(Co-IP).Results There was an interaction between Pax7 and Ubc9 in yeast.Pax7 can bind to Ubc9 specially in vitro.Pax7 could be SUMOylated in the presence of Ubc9 and it also could interact with SUMO.Conclusion The results demonstrate that Pax7 interacts with Ubc9,and the interaction leads to the SUMOylation of Pax7.
- Pax7 /
- Ubc9 /
- SUMO

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参考文献(10)

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