Abstract:
Objective To establish a loop-mediated isothermal amplification(LAMP) method for rapid detection of
Vibrio cholerae.
Methods Four primers were designed specifically according to
ctxA gene of
Vibrio cholerae.The LAMP system was developed and optimized.The specificity and sensitivity of this system were evaluated.Artificially contaminated fish and practical samples were detected.
Results The LAMP method was highly sensitive and specific.The sensitivity of the LAMP detection was 10 cfu/ml of bacteria samples and 10
2 cfu/g of
Vibrio cholerae in artificially contaminated fish.The assay identified 4
Vibrio cholerae,with
ctxA primers from 16 practical samples.
Conclusion A rapid,specific and sensitive LAMP method for detection of
Vibrio cholerae was established and could be used in rapid detection of
Vibrio cholerae in food samples.