大连地区HIV-1 CRF01_AE亚型株基因特征分析

刘大鹏; 吕秋月; 张群; 孙茂利; 任月; 赵志杰; 李德钧

doi:10.11847/zgggws2016-32-02-29

大连地区HIV-1 CRF01_AE亚型株基因特征分析

doi: 10.11847/zgggws2016-32-02-29

大连市疾病预防控制中心, 辽宁 116021

详细信息

作者简介:
刘大鹏(1978-),男,辽宁大连人,副主任技师,硕士,研究方向:HIV实验室检测及分子流行病学。

通讯作者:
李德钧,E-mail:ldjvis@163.com

中图分类号: R
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出版历程
- 接收日期: 2014-08-12
- 刊出日期: 2016-02-10

Genetic and epidemiological characteristics for HIV-1 CRF01_AE strains isolated in Dalian city

Dalian Municipal Center for Disease Control and Prevention, Dalian, Liao-ning Province 116021, China

摘要

摘要: 目的了解大连地区HIV-1 CRF 01_AE亚型株的基因特征及其可能传播链。方法对2008-2012年新确认144份全血标本进行血浆分离和HIV RNA提取,巢式PCR扩增Env基因并进行序列测定,并对CRF 01_AE亚型毒株序列进行生物信息学分析。结果得到的HIV感染者有效基因序列中, CRF 01_AE 亚型占62.14%(64/103),所有病例均为性接触途径传播。系统进化树分析大连CRF 01_AE存在2个簇,分别以男男同性传播和异性传播毒株为主,其中男男同性性接触人群(MSM)簇组内平均基因离散率小于异性性接触者(Hetero)簇;与其他地区的CRF 01_AE亚型株构建的系统进化树显示2簇内均无其他国家序列,MSM簇与辽宁(除大连地区,下同)、北京和吉林MSM人群的CRF 01_AE亚型株位于同一分支,Hetero簇毒株则与辽宁、北京的MSM流行株及东南沿海地区异性传播CRF 01_AE亚型株遗传距离相近。结论大连市HIV-1 CRF 01_AE株主要通过性接触传播,男男同性传播毒株占主导优势,毒株传入大连时间晚于异性性接触者簇流行株;MSM存在2个以上传播链,与北方部分地区MSM人群中CRF 01_AE亚型株存在多次传播。
- HIV-1 /
- CRF 01_AE亚型 /
- 进化分析 /
- 流行特征
Abstract: Objective To analyze genetic characteristics and transmission patterns of human immunodeficiency virus type1(HIV-1) CRF01_AE strains isolated in Dalian municipality in recent years.Methods Viral RNA was extracted from plasma samples,HIV-1 genes(env) were amplified by reverse transcriptase and nested-PCR using specific primer pairs.PCR products were sequenced and analyzed with phylogenetic method.Results A total of 26(63.41%) HIV-1 subtype CRF01_AE sequences were identified from the 41 samples successfully sequenced.All of the HIV/AIDS patients positive to CRF01_AE strains were infected through sexual contacts.Two main epidemic clusters,the men who have sex with men(MSM) cluster and the heterosexualit(Hetero) cluster(infected through heterosexual contact),were identified in the CRF01_AE strains in Dalian.Genetic distance analysis showed that the diversity of MSM cluster was obviously less than that of Hetero cluster and it was found that no international reference strain was closely correlated with the two clusters.The MSM cluster presented close relations with MSM strains form Liaoning province,Beijing and Jilin province;the Hetero cluster presented the close relations with homosexual strains from Liaoning province,Beijing and heterosexual strains from Southeast Coastal provinces.Conclusion The CRF01_AE strains are the dominant subtypes and homosexual transmission is the major route of transmission of the strains isolated in Dalian.The CRF01_AE strains of Dalian originated in Thailand and there were two or more transmission patterns in Dalian,and seemed to have had several events of epidemics in populations with the CRF01_AE strains isolated in MSM of northern region of China.
- HIV-1 /
- CRF01_AE /
- subtypes /
- phylogenetic analysis

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