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高煜欣, 李曦亮, 黄天壬, 邓伟, 郑丹, 李召发. 扶绥县肝癌高发区HBV流行株基因参照序列建立[J]. 中国公共卫生, 2016, 32(5): 687-690. DOI: 10.11847/zgggws2016-32-05-35
引用本文: 高煜欣, 李曦亮, 黄天壬, 邓伟, 郑丹, 李召发. 扶绥县肝癌高发区HBV流行株基因参照序列建立[J]. 中国公共卫生, 2016, 32(5): 687-690. DOI: 10.11847/zgggws2016-32-05-35
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GAO Yu-xin, LI Xi-liang, HUANG Tian-ren.et al, . Establishment of consensus sequences of Pre-S, S, Pre-C/BCP gene of hepatitis B virus prevaling in areas with high hepatocellular carcinoma incidence in Fusui county of Guangxi[J]. Chinese Journal of Public Health, 2016, 32(5): 687-690. DOI: 10.11847/zgggws2016-32-05-35
Citation: GAO Yu-xin, LI Xi-liang, HUANG Tian-ren.et al, . Establishment of consensus sequences of Pre-S, S, Pre-C/BCP gene of hepatitis B virus prevaling in areas with high hepatocellular carcinoma incidence in Fusui county of Guangxi[J]. Chinese Journal of Public Health, 2016, 32(5): 687-690. DOI: 10.11847/zgggws2016-32-05-35
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扶绥县肝癌高发区HBV流行株基因参照序列建立

Establishment of consensus sequences of Pre-S, S, Pre-C/BCP gene of hepatitis B virus prevaling in areas with high hepatocellular carcinoma incidence in Fusui county of Guangxi

    摘要
  • 摘要: 目的 建立广西扶绥县乙型肝炎病毒(HBV)流行株Pre-S、S、Pre-C/BCP基因参照序列。方法 选取2013年扶绥县乙肝慢性携带者(AsC)89例,测定其Pre-S、S、Pre-C/BCP基因,采用同源性分析等方法确定基因型,并按基因型在MEGA软件中进行序列比对,建立参照序列。结果 扶绥县参照序列与GenBank收录的中国其他地区同基因型HBV序列(CDQ448627、EU439006)相比差异较大,最高可达2.07%;扶绥县不同基因型间参照序列的差异程度大于不同地区同基因型序列间的差异,最小为2.87%,最大者可达9.65%。结论 初步建立扶绥县HBV流行株Pre-S、S、Pre-C/BCP基因参照序列,为扶绥县HBV基因突变的研究奠定基础。

     

    Abstract: Objective To establish consensus sequences of Pre-S, S, and Pre-C/BCP gene of hepatitis B virus(HBV) isolated in Fusui county of Guangxi Zhuang Autonomous Region(Guangxi).Methods Genes of Pre-S, S, and Pre-C/BCP of HBV isolated in 89 asymptomatic carrier of HBV were sequenced and the genotypes of the genes were determined with homology analysis.Consensus sequences of HBV prevailing in Fusui county were established base on different genotypes.Results Compared with the genotype sequences of HBV(CDQ448627, EU439006) isolated in other parts of China recorded in GenBank, the consensus sequences of Fusui county are of great difference, with a maximaum variation of 2.07%.The difference in the consensus sequences between different genotypes of HBV isolated in Fusui county was greater than the difference between the HBV isolated in different areas, with a minimum variation of 2.87% and a maximum of 9.65%.Conclusion The consensus sequences of Pre-S, S, and Pre-C/BCP gene of HBV isolated in Fusui county were primarily established and the study lays a foundation for researches on genetic mutations of HBV in Fusui county.

     

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