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王凤婕, 林健燕, 罗兰, 吴磊, 谭晓东. 彗星实验检测吸毒人群淋巴细胞DNA[J]. 中国公共卫生, 2005, 21(1): 35-36.
引用本文: 王凤婕, 林健燕, 罗兰, 吴磊, 谭晓东. 彗星实验检测吸毒人群淋巴细胞DNA[J]. 中国公共卫生, 2005, 21(1): 35-36.
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WANG Fengjie, LIN Jianyan, LUO Lan, . Monitoring DNA damage in human lymphocyte cells of drug-users with comet assay[J]. Chinese Journal of Public Health, 2005, 21(1): 35-36.
Citation: WANG Fengjie, LIN Jianyan, LUO Lan, . Monitoring DNA damage in human lymphocyte cells of drug-users with comet assay[J]. Chinese Journal of Public Health, 2005, 21(1): 35-36.
shu

彗星实验检测吸毒人群淋巴细胞DNA

Monitoring DNA damage in human lymphocyte cells of drug-users with comet assay

    摘要
  • 摘要:
      目的   应用彗星实验方法对吸毒人群淋巴细胞DNA损伤情况进行检测。
      方法   在湖北省某市戒毒所随机抽取123名吸毒人员作为暴露组, 同时选择79名非吸毒者作为对照组, 进行彗星实验。
      结果   暴露组和对照组的彗星细胞拖尾率分别为10.03%和1.26%, 差异有统计学意义; 两组的彗星细胞拖尾的尾长分别为(2.35±0.88)和(2.05±0.76)μm, 差异有统计学意义; 吸毒年限长者彗星细胞拖尾率(11.49%)显著高于吸毒年限短者(2.98%).多因素非条件Logistic回归分析结果表明, 吸毒人群DNA损伤的可能性明显高于对照组。
      结论   吸毒对人体淋巴细胞DNA有一定损伤, 吸毒时间越长, DNA损伤越严重。

     

    Abstract:
      Objective   DNA damage in human lymphocyte cells of drug-users was monitored with comet assay.
      Methods   123 drug-users were randomly selected as exposure group from a detoxification setting in Hubei and 79 not exposed to drug as control group.DNA damage in their lymphocyte cells was detected with comet assay.
      Results   Rate of DNA tail was 10.03% in exposure group, significantly higher than that in control group(1.26%), with very statistical significance, and tail length was(2.35±0.88)min exposure, significantly higher than that in control group(2.05±0.76)um.Rate of DNA tail was 1149% in exposure group with long length of drug-addicts, significantly higher than that with short length of drug-addicts(2.98%).Multivariate unconditional logistic regression analysis showed that possibility of DNA damag e was significantly higher in exposure group than that in control group(P < 0.05).
      Conclusion   Drug-addicts could cause certain damage to human lymphocyte cell DNA, the longer time of drug-addicts, the more serious damage of DNA.

     

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