富铬酵母液的提取及氨基酸含量分析

Analysis on amino acids of chromium-rich brewer's yeast

摘要:
目的探索从富铬酵母中提取葡萄糖耐量因子(GTF)的方法, 分析提取液中氨基酸含量。
方法应用萃取和超滤的方法从富铬酵母中提取富铬酵母液, 使用氨基酸自动分析仪检测提取液中氨基酸含量。
结果提取液中含有丰富的蛋白和氨基酸, 其中被认为构成葡萄糖耐量因子的谷氨酸、胱氨酸与甘氨酸的含量均较高, 分别为3878, 670与424mg/L。
结论应用超滤的方法提取的提取液中含有丰富组成GTF成分的氨基酸。

Abstract:
Objective To study method of GTF abstracted from chromium-rich brewer's yeast, determine content of amino acid in abstract.
Methods Extraction and ultrafilitraction were used to obtain abstact from chromium-rich brewer's yeast.
Results Many kinds of amino acids were determined in abstract. Content of glutamine, glycine and cystine was high (3878, 670, and 424 mg/L)。
Conclusion Concentration abstrated by eutraction and ultrafitration contained lots of amino acids which were the content of GTF.