夹心免疫PCR方法检测金葡菌肠毒素B

Establishment of sandwich immuno-PCR technique for detection of SEB

摘要:
目的建立双抗夹心免疫PCR的检测技术, 用于检测极微量的抗原。
方法以亲和素作为连接分子, 连接生物素化抗体和生物素化DNA, 应用于免疫PCR中检测极微量金黄色葡萄球菌肠毒素B (SEB)。
结果双抗夹心免疫PCR的检测技术, 检测SEB的灵敏度达0.1ng/L, 与夹心ELISA方法比较灵敏度高10³倍。
结论双抗夹心免疫PCR系统灵敏度高, 可用于各种微量抗原的检测。

Abstract:
Objective To develop sandwich immune-PCR system for monitoring antigen.
Methods Avidin was used to bridge the biotinylated antibodies and biotinylated DNA, and to detect antigen by immuno-PCR.
Results As little as 0.1ng/L of SEB could be detected by the sandwich immuno-PCR, which was 10³ times more sensitive than ELISA parallel control.
Conclusion The universal immuno-PCR system can be used to detect ultra-micro antigen.