慢性染铅对海马CA1区LTP及ERK2活性影响

杨菁; 孙黎光; 宗志宏; 蔡葵; 杜玉鑫

慢性染铅对海马CA1区LTP及ERK2活性影响

Effect of chronic lead exposure on rathippocampal CA1 LTP and ERK2 activity

摘要

摘要:
目的探讨慢性染铅对海马CA1区LTP及ERK2活性的影响.
方法用同心圆电极刺激海马的Schaffer侧支, 于CA1区用细胞外玻璃电极记录单脉冲刺激引起的群峰电位(PS), 观察对照组和染铅组大鼠于高频刺激后PS幅值的变化; 同时利用Western Blots方法检测海马CA1区ERK2的活性.
结果 HFS前记录的对照组和染铅组的PS幅值无显著性差异; HFS后染铅组的PS平均幅值与对照组相比下降了79%(P < 0.01);染铅组CA1区的ERK2的活性比对照组下降了24.1%(P < 0.01).
结论慢性染铅可抑制CA1-LTP的形成, 同时这种抑制与铅对ERK2活性抑制密切相关.

Abstract:
Objective To investigate the effects of chronic lead exposure on rathippocampal CA1LTP and Extra Cellular Signal Regulated Kinase 2(ERK 2)activityin vivo.
Methods A stimulus bipolar electrode was placed in the Schaffer/Commissural fibers, with extra cellar microelectrode technique recorded Population Spike(PS)in CA1 pyramidal, and the changes of PS amplitude was observed before and after the high frequency stimulation(HFS)of lead exposure group and control, respectively.The hippocampal CA1 activity of ERK 2 was determined by Western blots.
Results the baseline recordings of PS evoked by single pulse stimulation revealed no differences between control and lead exposure group.After HFS, the PS of lead exposure, on an average, decrease 79% compared with the control(P < 0.01).Lead exposure hippocgmpal CA1 ERK 2 activity decreased 24.1% compared with the control(P < 0.01).
Conclusion Chronic lead exposure inhibited CA1 LTP in vivo, and the decrease of RRK 2 activity may play an important role in this inhibition.

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