马齿苋抗家兔主动脉泡沫细胞形成机制的探讨
Study on mechanism of purslane on anti-aorta foam cells forming
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摘要:目的 探讨马齿苋抗家兔主动脉泡沫细胞形成的作用机制, 研究影响动脉粥样硬化(AS)形成的有关因素.方法 动物分为4组, A、B、C3组建立动物高脂模型, B、C组分别喂饲马齿苋干粉8或12g/(d·只), 实验11周末, 采血测定血脂、丙二醛、血液粘度等指标并观察透射电镜下主动脉内膜病理改变.用多元逐步回归分析, 筛选影响AS形成的主要因素.结果 B、C组与A组相比, 血清总胆固醇(TC)、甘油三脂(TG)、丙二醛(MDA)、全血低切表观粘度、血浆中切表观粘度水平明显下降(P < 0.01);高密度脂蛋白胆固醇(HDL-C)明显上升(P < 0.01), C组TC、全血低切表观粘度数值较B组明显降低(P < 0.01).多元方程显示α=0.05, 0.01时对Y有显著影响的是TC(X1), 血浆中切表观粘度(X6).结论 马齿苋可明显阻止或降低血清TC、TG、MDA、全血低切及血浆中切表观粘度的升高, 并升高HDL-C, 随着剂量的增加, 马齿苋作用增强.电镜观察显示马齿苋能使主动脉内膜泡沫细胞数目减少, 脂质沉积减轻, 抑制AS斑块形成.多元分析表明, 影响主动脉AS形成的主要因素是TC及血浆中切表观粘度, 二者皆为正向影响, 且TC影响最大.试验显示, 马齿苋降低该两项因素的作用最为明显.Abstract:Objective To explore the mechanism of purslane on anti-aorta foam cells forming and to study various factors in affecting AS forming.Methods Rabbits were divided into 4 groups.The model of hyperlipidemia was established in A, B, C groups.B, C groups were given 8 or 12 gram purslane every day to each animal for 11 weeks.Blood lipids, molondiadehyde(MDA)and blood viscosity were determined.Aor at intima pathological changes under the electronmicroscope was observed.Stepwise regr ession was used to siev e primary factor in affecting of AS forming.Results Serum cholesterol(TC), triglyleride(TG), MDA, whole blood and plasma viscosities in B, C groups were markedly decreased compared to A group(P < 0.01);buthDL-C was obviously increased(P < 0.01).TC, whole blood viscosity in C group were significantly lower compared with B group(P < 0.01).Conclusion Purslane powder could markedly restrain or lower TC, TG, MAD, whole blood and plasma viscosity; but raise HDL-C.Accompany ing dose increase, puslane effect was increased.It was proved under the observation of electronmicroscope that purslane could decrease lipids deposition of arotoaintima and inhibit the formation of ather osclerotic plagul.Multiple stepwise regression analysis suggested that TC and plasma viscosity were primary factors in influence of AS forming.Purslane can markedly decreased TC and plasma viscosity.
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