不同热处理时大鼠血清急相反应蛋白的变化

宋剑锋; 戴军; 陈胜; 王瑞波; 邬堂春

不同热处理时大鼠血清急相反应蛋白的变化

Change of APRPs in sera of rats with differentheat treatments

摘要

摘要:
目的探讨大鼠在不同热处理条件下几种急相反应蛋白(APRPs)的变化.
方法 SD雄性大鼠60只分为对照组、热接触组、热衰竭组、预热处理组, 分别给予不同的热处理, 采用琼脂糖火箭免疫电泳、免疫扩散法测定血清铜兰蛋白(CP)、触珠蛋白(HP)、纤维连接蛋白(Fn)的含量.
结果热接触组、热衰竭组及预热处理组CP含量较对照组明显升高(P < 0.05), 且预热处理组较热接触组、热衰竭组明显升高(P < 0.05);热接触组、预热处理组HP含量较对照组及热衰竭组明显升高(P < 0.05);热衰竭组Fn含量较对照组、热接触组、预热处理组的含量明显下降(P < 0.05).
结论 CP、HF、Fn是反映热损伤较为敏感的指标, 其含量增高可被看作机体保护性反应增高的一种征象, 同时, Fn含量下降可能提示急性受热对机体造成了一定损伤.

Abstract:
Objective To study the changes of several kinds of APRPs of rat with differentheat treatments.
Methods 60 male SD rats were randomly divided into control group, heat exposure group, heat exhaustion group and preheat treating group.Using rocket immunoelectrophoresis and radial immunodiffusion, the contents of ceruloplasmin(CP), haptoglobin (HP), fibronection(Fn)in sera were determined.
Results the contents of CP in all groups increased sig nificantly compared with the control group, and that in preheat treat group increased significantly compared with the heat exposure group or heat exhaustion group(P < 0.05);the contents of HP in heat exposure group, the preheat treating group increased significantly compared with the control group or the heat exhaustion group(P < 0.05);the contents of Fn in heat exhaustion group decreased significantly compared with the other three groups(P < 0.05).
Conclusion CP, HP, Fn were relatively sensitive biomarkers of heat; the increases in CP, HP might be regarded as a sign of self-defensive response, and the decrease in Fn sugg ested that rats were damaged by acute heat.

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