软骨藻酸对小鼠脑组织HO-1蛋白表达的影响
Effects of domoic acid on heme oxygenase-1 protein expression in mouse brain
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摘要:目的 研究软骨藻酸(domoicacid)对小鼠脑组织丙二醛(MDA)含量及血红素氧化酶-1(hemeoxygenase-1, HO-1)蛋白表达的影响.方法 小鼠连续5d腹腔注射软骨藻酸染毒后, 采用硫代巴比妥酸(TBA)法测定小鼠脑组织中MDA含量, 并用免疫组织化学方法检测小鼠脑组织中HO-1分布及表达.结果 低剂量(1/90LD50)组海马、高剂量(1/10LD50)组大脑皮层和海马MDA含量明显升高, 与对照组相比有显著性差异(P < 0.05).对照组大脑皮层和海马HO-1阳性细胞平均光度(A)分别为0.0953±0.0252和0.0940±0.0304;低剂量组皮层和海马A分别为0.1410±0.0407和0.1406±0.0354, 与对照组相比, 有显著性差异(P < 0.05);高剂量组大脑皮层和海马A分别为0.1626±0.0374和0.1604±0.0325, 与对照组相比, 有显著性差异(P < 0.01).结论 软骨藻酸对小鼠大脑皮层和海马具有氧化损害作用, 并诱导皮层和海马HO-1蛋白表达增多.Abstract:Objective To study the effects of domic acid on MDA content and heme oxygenase-1(HO-1)protein expression in mouse brain.Methods TBA method and immunity histochemistry staining were applied to assess the MDA content and HO-1 protein expression respectively in mouse brain after administration domoic acid, ip for 5 days.Results the MDA content in hippocampus in low dose group(1/90 LD50)and in high dose group(1/10 LD50)were significantly different from that in hippocampus in control group(P < 0.05).The MDA content in cortex of high dose group was significantly different form that in cortex in control group(P < 0.05).The mean light activity of positive HO-1 cells respectively was 0.141 0±0.040 7 in hippocampus and 0.140 6±0.035 4 in cortex in low dose group, respectively higher than 0.095 3±0.025 2 in hippocampus and 0.094 0±0.030 4 in cortex in control group(P < 0.05).The mean light activity of positive HO-1 cells respectively was 01162 6±0.037 4 in hippocampus and 0.160 4±0.032 5 in cortex in high dose group, respectively higher than that in hippocampus and in cortex in control group(P < 0.01).Conclusion Domoic acid could cause oxidative injury in hippocampus and cortex in mouse brain and increase HO-1 protein expression as well.
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