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张全武, 孙少华, 王振全. 甲醛对小鼠脏器MGMT的影响[J]. 中国公共卫生, 2004, 20(1): 61-62.
引用本文: 张全武, 孙少华, 王振全. 甲醛对小鼠脏器MGMT的影响[J]. 中国公共卫生, 2004, 20(1): 61-62.
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ZHANG Quan-wu, SUN Shao-hua, WANG Zhen-quan. Effects of formaldehyde on MGMT in mice organs[J]. Chinese Journal of Public Health, 2004, 20(1): 61-62.
Citation: ZHANG Quan-wu, SUN Shao-hua, WANG Zhen-quan. Effects of formaldehyde on MGMT in mice organs[J]. Chinese Journal of Public Health, 2004, 20(1): 61-62.
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甲醛对小鼠脏器MGMT的影响

Effects of formaldehyde on MGMT in mice organs

    摘要
  • 摘要:
      目的   研究甲醛对小鼠脏器O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)表达的影响.
      方法   选择健康昆明小鼠40只, 随机分为4组: 甲醛低剂量组(2.5mg/m3)、中剂量组(5mg/m3)、高剂量组(10mg/m3)、对照组(无甲醛暴露), 每组10只.除阴性对照组外, 其余3组每日吸入甲醛1次, 每次4h, 连续9周.应用链霉亲和素生物素-过氧化物酶(SABC)免疫组化法对小鼠脑、肺、肝及肾组织中MGMT进行检测.
      结果   3个染毒组小鼠肝和肺组织中MGMT表达降低, 与对照组比较具有显著性差异(P < 0.05或P < 0.01);脑和肾组织MGMT表达各组之间无显著性差异(P > 0.05).
      结论   甲醛对小鼠肝和肺组织MGMT表达可能具有抑制作用.

     

    Abstract:
      Objective   To study the effects of formaldehye(FA)on expression of MGMT in mice organs.
      Methods   40 healthy Kunming mice were selected and randomly divided into 4 groups, 10 mice per group, FA low-dose group(2.5 mg/m3), FA middle-dose group(5 mg/m3), FA hig h-dose group(10 mg/m3), experimental control group(exposed no FA).Except the control group, the other 3 groups were exposed to FA by inspiration in a static total enclosure chamber 4 hours once a day for 9 consecutive weeks.After exposure, the MGMT in brain, lung, liver and kidney was examined by SABC immuno histo chemical staining.
      Results   the expression of MGMT in liver and lung in all experimental groups were downregulated compared with the control(P < 0.05 orP < 0.01).MGMT expression of brain and kidney did not change after exposure to FA.
      Conclusion   FA could decrease the expr ession of MGMT in liver and lung in mice.

     

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