c9, t11-共轭亚油酸对巨噬细胞因子表达的影响

王璇琳; 陈炳卿; 赵秀娟; 杨艳梅; 郑玉梅; 刘家仁

c9, t11-共轭亚油酸对巨噬细胞因子表达的影响

Effects of c9, t11-conjugated linoleic acid on cytokine expression of macrophage

摘要

摘要:
目的研究c9, t11-共轭亚油酸(Conjugated linoleic acid, CLA)对C57小鼠巨噬细胞杀伤黑色素瘤细胞(B16-MB)能力的影响.
方法用0, 25, 50, 75, 100μmol/LCLA处理巨噬细胞24h后, 分别用MTT法检测CLA处理的巨噬细胞对B16-MB细胞的杀伤能力; RT-PCR方法检测C57小鼠巨噬细胞细胞因子IL-6、TNF-α和iNOSmRNA表达.
结果在100, 75μmol/LCLA处理后, 巨噬细胞对肿瘤的杀伤率分别为18%和14.5%: 同时, IL-6、TNF-α和iNOSmRNA表达增加.
结论 c9, t11-CLA增强C57小鼠巨噬细胞对B16-MB细胞的杀伤能力, 并与其诱导IL-6、TNF-α和iNOSmRNA的表达有关.推测CLA发挥抗肿瘤作用可能与其参与机体免疫调节作用有关.

Abstract:
Objective To study the effects of c9, t11-conjugated linoleic acid on the killing ability of macrophage to B16-MB cells in C57 mice and explore its possible mechanism.
Methods Five levels of CLA was designed as 0, 25, 50, 75, 100 μmol/L.After macro phage was treated with CLA for 24 h, the MTT assay was used to evaluate the killing ability of macrophage to B16-MB cells; the RT-PCR was used to detect the expression of macrophage cytokine IL-6, T NF-a and iNOS mRNA in C57 mice.
Results At the concentrations of 100, 75 μmol/L c9, t11-CL A, the cytotoxic effects of macrophage on tumor cells were 18% and 14.5%, respectiely.At the same time, the expression of IL-6, TNF-A and iNOS mRNA increased.
Conclusion c9, t11-CLA could increase the killing ability of macrophage to B16-MB cells in mice, and was associated with the induction of IL-6, T NF-A and iNOS mRNA expression.Antitumor ability of CLA may be asso ciated with taking part in immune regulation action.

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