茶多酚、维生素C对镉肾毒性影响的实验研究
Effects of tea polyphenols and vitamine C on Cd-induced nephrotoxicity
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摘要:目的 研究茶多酚(TP)、维生素C(Vc)对镉肾毒性的影响.方法 将Wistar大鼠40只, 随机分成4组.第1组为对照组, 第2组大鼠皮下注射144mg/kg氯化镉溶液; 第3、4组大鼠以TP、Vc预处理后皮下注射1.44mg/kg氯化镉溶液, 每周3次, 连续6周.分别在实验开始后第4周和6周时将大鼠移入代谢笼, 收集24h尿样, 分别测定尿N-乙酰-P-氨基葡萄糖酶(NAG)活性和尿蛋白、尿镉含量.于最后一次注射24h后处死大鼠, 采集血液和肾皮质样品.测定各组大鼠血清尿素氮、血及肾皮质的丙二醛(MDA)含量, 血及肾皮质镉含量.结果 染镉4周后, 与单纯染镉组比较, TP和Vc能明显降低尿NAG酶活性和尿蛋白含量.染镉6周后, TP和Vc能显著降低血清尿素氮、血及肾皮质MDA含量.TP还能显著降低血、肾皮质镉及升高尿镉含量.结论 TP、Vc预处理对镉中毒所致的肾损伤有一定的预防作用, 此种预防作用可能与它们的抗氧化作用及促进镉排泄有关.Abstract:Objective TP(Tea polyphenols)and Vc(Vitamine C)were given to rats to study the effects on the nephrotoxicity induced by chronic exposure to cadmium.Methods Four groups were used for the study.Three experimental groups were given subcutaneous injection of 1.44 mg/kg CdCl 2, two of which were pretreated by TP and Vc respectively before the injected of CdCl2, three times each week for 6 weeks.Control group was injection with 0.9% NaCl at the same time.Urinary samples were collected at the end of the 4th and 6th week.NAG activities, protein, and cadmium contents were determined in urine.Kidney and blood were collected 24 h later after the final injection.BUN and MDA in blood and renal cortex were mensuated.Results NAG activities and protein content in urine of TP and Vc groups decreased significantly compared with those of animals given cadmium alone at the end of week 4.After week 6 the pretr eatment of TP and Vc decreased BUN significantly in blood as well as MDA contents of renal cortex and blood.Moreover, TP obviously decreased Cd content in blood and renal cortex, but increased Cd content in urine.Conclusion TP and Vc could protect rats against the toxic effects of Cd on kidney, which may be the result of reducing lipid peroxidation and Cd content.
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