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余小平, 糜漫天, 朱俊东. 三羟异黄酮对乳腺癌异种移植瘤血管生成影响[J]. 中国公共卫生, 2003, 19(10): 1168-1170.
引用本文: 余小平, 糜漫天, 朱俊东. 三羟异黄酮对乳腺癌异种移植瘤血管生成影响[J]. 中国公共卫生, 2003, 19(10): 1168-1170.
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YU Xiao-ping, MI Man-tian, ZHU Jun-dong. Effect of genistein on angiogenesis in breast cancer xenograft tumor[J]. Chinese Journal of Public Health, 2003, 19(10): 1168-1170.
Citation: YU Xiao-ping, MI Man-tian, ZHU Jun-dong. Effect of genistein on angiogenesis in breast cancer xenograft tumor[J]. Chinese Journal of Public Health, 2003, 19(10): 1168-1170.
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三羟异黄酮对乳腺癌异种移植瘤血管生成影响

Effect of genistein on angiogenesis in breast cancer xenograft tumor

    摘要
  • 摘要:
      目的   探讨三羟异黄酮(genistein)对原癌基因(HER-2/neu)高表达乳腺癌血管形成的影响及其与血管内皮生长因子(VEGF)的关系。
      方法   应用基因转染技术建立HER-2/neu高表达的乳腺癌细胞(MCF-7/HER-2)。免疫缺陷裸鼠(BALB/c)皮下接种乳腺癌(MCF-7和MCF-7/HER-2)细胞, 其中接种MCF-7/HER-2细胞的裸鼠随机分为3组: 对照组; genistein处理组: HER-2/neu抗体处理组。应用免疫组化法观察移植瘤内微血管密度(microvesseldensity, MVD)和VEGF的表达变化。
      结果   MCF-7/HER-2移植瘤与MCF-7移植瘤相比血管密度较大, VEGF表达增强; MCF-7/HER-2移植瘤经genistein和HER-2/neu抗体处理后, 移植瘤的血管密度减少, VEGF表达降低。
      结论   HER-2/neu高表达能促进乳腺癌的血管生成, genistein可能通过下调VEGF的表达从而抑制HER-2/neu高表达乳腺癌的血管生成。

     

    Abstract:
      Objective   To explore the effect of genistein on angiogenesis in HER-2/neu-overex pressing breast cancer and the relationship with vascular endothelial growth factor.
      Methods   HER-2/neu-over expressing MCF-7 human breast cancer cells(MCF-7/HER-2)were generated by gene transfecting technique.MCF-7/HER-2 cells and MCF-7 cells were injected into BAL B/c nude mice. The nude mice with MCF-7/HER-2 xenograft were randomly divided into three groups: control group, genistein-treated group and HER-2/neu antibody-treated group. Microvessel density and VEGF expression exchang in xenograft tumors were analyzed with immunohistochemical staining.
      Results   Micro vessel density(MVD)and VEGF expression were significantly higher in MCF-7/HER-2 xenograft tumors than that in MCF-7 xenograft tumors.MCF-7/HER-2 xenog raft tumors treated by genistein or HER-2/neu antibody showed lower MVD and VEGF expression as compared with MCF-7/HER-2 xenograft tumors.
      Conclusion   The overexpression of HER-2/neu could promote angiogenesis in breast cancer, and genistein could inhibit angiogenesis in HER-2/neu-overexpressing breast cancer through down-regulation expression of VEGF.

     

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