共轭亚油酸抑制人胃腺癌细胞体外侵袭作用

杨艳梅; 陈炳卿; 王璇琳; 郑玉梅; 刘家仁; 刘瑞海

共轭亚油酸抑制人胃腺癌细胞体外侵袭作用

Effects of conjugated linoleic acid on invasion of human gastric carcinoma cell in vitro

摘要

摘要:
目的采用体外细胞培养技术, 模拟体内肿瘤转移过程, 研究c9, t11-共轭亚油酸(CLA)对肿瘤细胞侵袭及侵袭相关特性的影响。
方法用0, 25, 50, 100和200μmol/LCLA处理SGC-7901人胃腺癌细胞24h后, 分别进行重组基底膜侵袭试验、趋向性运动实验和粘附试验研究CLA对侵袭、运动和粘附能力的影响。
结果在200, 100和50μmol/L浓度时, c9, t11-CLA对肿瘤细胞侵袭重组基底膜的抑制率分别为53.7%, 40.9%和29.3%。同时, c9, t11-CLA处理后的肿瘤细胞与细胞外基质的粘附能力降低, 但对肿瘤细胞趋化运动能力的影响不明显。
结论 c9, t11-CLA可降低肿瘤细胞的体外侵袭能力, 改变某些侵袭相关性质。

Abstract:
Objective To investigate the effects of c9, t11-conjugated linoleic acid on cancer invasion and invision-related characteristics the metastasis cascades of tumor cells were imitated in cell culture.
Methods The five levels of CLA in medium were designed as 0, 25, 50, 100 and 200μmol/L. After human gastric carcinoma cell line SGC27901 were treated with c9, t11-CLA for 24 h, reconstituted basement membrane invasion assay and migration assay was used to evaluate invasive ability and migration ability of cancer cells, adhesion assay was investigated the ability of treated cells to adhere to intracellular matrix.
Results At the concentrations of 200, 100 and 50μmol/L, c9, t11-CLA suppressed the reconstituted basement memebrane invasion of SGC-7901 by 53.7%, 40.9% and 29.3%.Meanwhile, c9, t11-CLA could inhibit the adhesion to laminin, fibronectin and matrigel.c9, t11-CLA did not, however, significantly influence the migration ability of treated cells.
Conclusion c9, t11-CLA could suppress invasion of SGC-7901 cells and change some invasion2related characteristics.

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