Aγ-珠蛋白基因DNA多态性研究

DNA-polymorphism in Aγ-globin

摘要:
目的研究Aγ-珠蛋白基因的多态性。
方法采用PCR-RFLP技术对35例日本人Aγ-珠蛋白基因领域DNA多态性进行了检测。
结果 Aγ-Gg ^*1纯合子表型频率为0.1143, Aγ-Gg ^*2纯合子表型频率是0.2857, Aγ-Gg1/Aγ-Gg ^*2杂合子表型频率是0.60, Aγ-Gg ^*1基因频率0.4143, Aγ-Gg ^*2基因频率0.5857, 而所有基因座经检验均符合Hardy-Weinberg平衡。
结论该扩增体系在日本人群等位基因分布较好。

Abstract:
Objective To study DNA-polymorphism in the AC-globin.
Methods The PCR-RFLP was adopted to study 35 Japanese DNA-polymorphism of AC-globin.
Results AC-Gg ^*1 homozy gote phenoty pefrequency was 0.114 3, AC-Gg ^*2 homozygote phenot ype frequency was 0.285 7, AC-Gg ^*1/AC-Gg ^*2 heterozygote phenotype frequency was 0.60, AC-Gg ^*1 gene frequency was 0.414 3, AC-Gg ^*2 gene frequency was 0.585 7 and gene sites suited to Hardy-Weiner gequilibrium.
Conclusion This augmentation system well distributed in the Japanese population.