Abstract:
Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.
Methods A diagnostic kit(FQ-PCR)was developed according to the common sequence of NhaA gene in vibrio cholerae.The sensitivity and speciality in detecting of vibrio cholerae in infectious water, sea food and winter-endurance specimen and clinical samples were compared with FQ-PCR kit.
Results In the detection of clinical samples, FQPCR and regular detection ways have the same sensitivity and speciality.While in the detection of infectious water, sea food and winter-endurance specimen, FQ-PCR has superiority to regular ways.
Conclusion The construction of FQ-PCR method eliminates PCR cross-contamination which cause false positive, and real-time detection ensure accurate quantity.So FQ-PCR can be used to the detection of vibrio cholerae in clinical and sanitary laboratory.
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