Abstract:
Male wistar rats w ere eanployed in the present study.They were admistered successively 10mg methylmerculy(CH
3HgCl)/Kg weghtby subcutaneouinyctinn for 7 days On 15th day the brain tissues of rats w ere removed and DNA electrophoresis and analysis of flow crytcxn etry were perforned by malong use of tec;hnologis on clhzlar andm olecular biobgy. DNA was extracted from brain tissues ofCH
3HgCl-treatedrats A typi;alladder of DNA fragnments was seengel electrophoresis Neurons apoptosis rate and expression level of P
53 gene from CH
3HgCl-treated g mup were obviously higher thanthatfrom cnntrolgroup by analysis offlow cytome try(P<0.05). The experin ental results demonstrate that CH
3HgCl causes injury of brain neuron by inducing apoptosis P
53 gene participates in gene regulation in process of CH
3HgCl )-induced apoptos is of hrain neurons.