免疫酶斑点试验检测病人粪便中轮状病毒

Detection of Rotaviruses in Feces of Patients by Immunoenzyme Spot Assay

摘要: 人轮状病毒（HRV）是引起婴幼儿急性胃肠炎的主要病原。本实验室用免疫酶斑点法（Immune Enzyme Dot Assay）测定HRV抗原并与常规的ELISA、电镜（EM）作了对比，实验结果证明IEDA具有敏感、快速、简易等优点。且不需大的实验设备，具有很大的实用价值。

Abstract: Immunoenzyme spot assay (IESA） Was carried out on the filter membrane of nitro-cellulose.The principle and the process of operation of this method are similar to those of ELTSA except the difference is the solid phase used.We have determined 38 stool specimens collected from diarrheal infants by indirect IESA and ELISA.The results showed that this IESA is simple,inexpensive,easy to reproduce and no special epuipment was needed and can be completed in 2.5 hours.There was no significant difference between ELISA and IESA (X²=1.12,P>0.05） in examination of rotaviruses in feces.