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2005 Vol. 21, No. 10

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Study on hair trace elements levels of pregnant women from birth defects surveillance system
ZHANG Wei, REN Aiguo, PEI Lijun,
2005, 21(10): 1153-1155.
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  Objective   To explore early pregnant women's hair trace elements distribution status between high and low birth defects incidence areas, and to provide primary clues for birth defects risk factor study.   Methods   Using the International Atomic Energy Agency(IAEA)recommended hair washing procedure, microwave digestion methods and ICP-MS detection instrument, an ecological study was carride out to detect the levels of six necessary trace elements, that were recruited from the southern urban, southern rural, northern urban and northern rural areas of China-US birth defect surveillance system. Confounding factors including days of pregnancy, mothers' age, hair washing intervals, smoking and drinking habits were collected by questionnaire.   Results   Geometric means of hair zinc levels for southern urban, southern rural, northern urban and northern rural areas were 220.60, 218.62, 210.01 and 186.46(μg/g)respectively. Statistical differences for hair zinc levels were found between southern and northern, urban and rural areas(P < 0.01) as a whole, but the differences only existed between northern urban and northern rural areas(P < 0.001), and no difference was found between southern urban and southern rural areas. Multi-variable analysis results showed that the living area was one of the most important factors for hair elements levels.   Conclusion   Hair trace elements levels are different among different birth defect areas, and the distribution tendency for pregnant women's hair zinc average level is similar to that of the neural tube defects incidence in different areas. This reminds us that zinc may be related to neural tube defects.
Effects of antenatal and postnatal lead exposure on infants physical development
SHEN Tong, ZHU Zhongping, YU Cuilian,
2005, 21(10): 1156-1158.
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  Objective   To explore the effect of antenatal and postnatal lead exposure on infants physical development in lead polluting area.   Methods   Prospective cohort study was performed. The newly-wedding couples cohort was found in lead polluting area. Baseline investigation, pre-pregnacy observation, pregnant period follow-up and labour and newborn baby record among them. When their offspring was infant period, physical examination were carried out. Meanwhile, blood were drawn and blood lead levels were measured with atomic absorption spectrophotometry.   Results   The difference of Z-standard score for infant height was significant between the high and low lead of mother baseline blood, Z-standard score for infant chest circumferenc in mother and father baselin blood. The difference of height growth rate within 24 months was significant between the high and low lead of mother and father baseline blood, umbilical cord blood. There were negative relation between blood lead levels and Z-standard score and height growth rate.   Conclusion   Infants physical development retard caused by lead exposure is associated with antenatal and postnatal exposure even baseline lead load in parent. Prevention of child lead poisoning should begin from antenatal.
2005, 21(10): 1158-1160.
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2005, 21(10): 1160-1161.
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2005, 21(10): 1162-1163.
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2005, 21(10): 1163-1163.
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2005, 21(10): 1164-1164.
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2005, 21(10): 1165-1165.
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Genetic association between polymorphism of claudin 5 transmembrane protein gene and schizophrenia
XIE Lin, JU Guizhi, SHI Jieping,
2005, 21(10): 1166-1167.
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  Objective   To investigate the association between schizophrenia and the polymorphism of claudin 5 transmembrane protein deleted in velocardiofacial syndrome(CLDN5).   Methods   PCR-Restrictive Fragment Length Polymorghism was used to examine the G/C single nucleotide polymorphism(SNP)of CLDN5 with 173 muclear family trios of schizophrenia.   Results   The result of TDT indicated that the allelic frequency transmitted from the heterozygote parents didn't deviate 50%;There was significant relationship between CLDN5 genotype and delusion of influence(P < 0.05).   Conclusion   As far as Chinese han schizophrenia in this paper are concerned, the association between the polymorphism of CLDN5 and schizophrenia may not be assured, but the effect of polymorphism of CLDN5 on psychotic symptoms in schizophrenia cann't be exculded.
Effects of dibutyl phthalate on testosterone biosynthesis in rats
WANG Yubang, SONG Ling, ZHU Zhengping,
2005, 21(10): 1168-1170.
Abstract(968) HTML (238) PDF 479KB(73)
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  Objective   To investigate the effects of dibutyl phthalate(DBP)on testosterone biosynthesis and its related mechanism in rats.   Methods   Six-week-old healthy male SD rats were randomly divided into 4 groups with 8 animals per group. DBP dissolved in peanut oil was administered by gavage at doses of 0, 250, 500, 1 000 mg/(kg·d). After 4-week DBP exposure, the animals were sacrificel with testis selected and weighed. Luteinizing hormone(LH), 17β estradiol(17β-E2), testosterone(T)were detected by radioimmunoassay. The relative expression levels of cholesterol sid-echain cleavage enzyme(P450scc)mRNA and 3beta-hydroxysteroid dehydrogenase(3β-HSD)mRNA were determined by RT-PCR.   Results   LH levels in serum were not significantly changed, whereas 17βE2 in serum elevated noticeably induced by DBP. T levels in testis homogenate and in serum reduced signifcantly in 500 and 1 000 mg/(kg·d)groups. Furthermore, mRNA expression was downregulated for P450scc and 3β-HSD following DBP exposure. Linear correlation and regression revealed that there were obvious relationships between dosages(logarithm)and the expression of P450scc mRNA and 3β-HSD mRNA.   Conclusion   Leydig cells may be one of the major target cell affected by DBP. It is likely that downregulation of T biosynthetic enzymes mRNA may be key factors contributing to the decrease of T concentration. Secondly, The increase of 17βE2 disordered physiologic balances of hypothalamic-pituitary-testis axis(HPTA)and local modulation in testis.
Effects of iodine deficiency on proten kinase C in developing rat hippocampus
YIN Hongbo, LIU Changsheng, WANG Ping,
2005, 21(10): 1170-1171.
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  Objective   To study the effects of iodine deficiency and hypothyroidism on activity of protein kinase C(PKC)in rat pups hippocampus.   Methods   Iodine deficient rats were administered with iodine-deficient diet and hypothyroid rats were administered with methimazole in drinking water. On postnatal 30 days, the hippocampus from the three groups was separated from the brain for PKC activity detection.   Results   Cytosol PKC activities in iodine deficient and hypothyroid groups(24.876 3±10.443 3)were higher than that of the control group(20.559 8±6.312 1)pmol/(minºmg). PKC activities(membrane/cytosol)in iodine deficient(1.187 1±0.432 5)and hypothyroid(1.414 3±0.394 0)groups were significantly higher than that of the control group(0.6493±0.294 3)(P < 0.01).   Conclusion   Iodine deficiency and hypothyroidism during critical periods brain developent may promote translocation of PKC from the cytosol to the membrane fraction and contribute to cognitive deficits associated with developmental iodine deficiency and hypothyroidism.
Study on polymorphism of 16s-23s rRNA ribosomal-spacerregion of Brucella in China
WANG Wenjing, RUI Yongyu, CHEN Yao,
2005, 21(10): 1172-1173.
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  Objective   To identify Brucella spp by 16 s-23 s rRNA Ribosoma-l Spacer-Region(ITS)and to estimate the significance of ITS as gene labelling in Brucella.   Methods   Strain typing of 120 Brucella spp. in China by PCR-SSCP analysis and sequencing for ITS.   Results   Four types(Ⅰ, Ⅱ, Ⅲ, Ⅳ)existed in all species which were examined. Sequence analysis of the ITS revealed a spacer of 765 bp with high(99.87%)homology and only 3 sites were different.   Conclusion   ITS with high homology is of some importance to be marker in Brucella spp.
Influenza activity and antigenic characterization in Guangdong in 2003
YAN Xinge, PENG Guowen, ZHANG Xin,
2005, 21(10): 1174-1175.
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  Objective   To study influenza activity and antigenic characterization in Guangdong during 2003.   Methods   The data of influenza surveillance were utilized. Antigenic analysis was performed by using cross hemagglutination inhibition test. The gene of hemagglutinin(HA1)protein were amplified by RT-PCR. PCR products were purified and sequenced. Finally, the sequence data were analyzed with Megalign and Editseq software.   Results   Of the 510 positive isolates, 481(9214)were in fluenza A(H3N2)viruses, 29 (7.6%)were influenza B viruses.52 outbreaks were confirmed by lab, including 50 due to influenza A(H3N2)viruses and 2 due to influenza B viruses. Most of influenza A(H3N2)viruses were similar antigenically to A/Fujian/411/02(H3N2), a drift variant of vaccine strain A/Panama/2007/99(H3N2). There were 25 different amino acid on HA1 domain and the homology of amino acid sequence was 92.1% when compared current strains in Guangdong with waccine strain A/Panama/2007/99(H3N2);28 of the influenza B viruses belonged to the B/Ⅴ ictoria lineage and were similar antigenically to the vaccine strain B/HongKong. 1 influenza B virus belonged to the B/Yamagata.   Conclusion   In Guangdong, influenza activity in 2003 was stronger than that in 2002;influenaz A(H3N2)and two lineages of influenza B were co-circulating. The predominant viruses were subtype of influenza(H3N2). And the antigenic drift of influenza(H3N2)occurred.
Effect of different calcium load on growth and lever of Ca, Fe, Zn in rats
YAN Chunsheng, LIU Hui, WANG Zhifan,
2005, 21(10): 1176-1178.
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  Objective   To Study the influence of excessive calcium supplyment on the growth of rats, metabolize of calcium and lever of iron and zinc.   Methods   40 weaned Wistar rats were divided randomly and equally into 4 groups according to the weight. One of them was background group which was killed before fed. The others were fed by different calcium content for 28 days then were engaged in metabolize of calcium experiment.   Results   Rats' growth were influenced by the different calcium content, which inclued rats' weight gained slowly. The weight of the thighbone was increased but the length of it wasn't increased. The bone were calcified too early by excessive calcium intake. Excessive calcium intake made the viscera coefficient of rats changed, and had certain harm to testicle and kidney and so on. The absorbability and using of calcium were decreased, which wasted calcium and increased metabolizable burden of body. At the same time, it made the concentration of serum zinc and serum iron depressed, but the serum calcium went up. High uric calcium was the latent dangerous factor of nephrolith.   Conclusion   The excessive calcium influenced the growth of rats and harmed certain viscera. And it disturbed the absorbability and using of calcium, iron and zinc.
Effect and mechanisn of Reduping on heat stroke in mice
YANG Guang, LUO Bingde, LI Wen,
2005, 21(10): 1178-1179.
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  Objective   To investigate the effect and mechanism of Reduping, a novel endotox in-antagonist made from traditional Chines medical herds, in the treatment of heat stroke and to provide experimental evidence for the prevention and cure of the disease.   Methods   The heat stroke model was established under circum stances of dry buld temperature(34.5±0.15)℃ and(60±5)% relative humidity. 40 mice were randomly divided into five groups including reduping group, xihuangqijiao group, Dexamethasone group, heat strike group and normal group and the increased rate of temperature, subsistence time and the number of diarrhea of experimental animals were observed.   Results   It was observed clearly that the increased rate of temperature, survival time the number of diarrhea of Reduping group were the slowest, longest and most among the these groups respectively.   Conclusion   Reduping have the better effect of prevention and cure to the heat stroke and one of the most important mechanisms is to improve intestinal function and to excrete more poisonous substances of body.
Occupational harmfulness of powder dust and control technology in coal preparation plant
LIU Xiaozhen, WAN Guo lin, HUANG Hai chao,
2005, 21(10): 1180-1181.
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  Objective   To find out the key control points and to put forward suitable control techology by investigating occupational harmfulness of powder dust in coal preparation plant.   Methods   The Rule of hazard analysis and critical control point(HACCP)was used in the research program. Field researches of technological process and working environment in coal-cleaning plants were surveyed. And the factors of occupational harmfulness of all working posts were identified. Based on the serious degree of occupational harmfulness from the concentration & dispersal degree of powder dust and the health damage of workers, key control points were found out and prevention methods were advanced.   Results   More harmfulness happened on the positons of crashing, filtering, loading and unloading workshop. Powder dust harmfulness in coal preparation plant mainly came from coal dust. The mean concentration of free SiO2 coal dust was 4.9%. The concentrations of coal powder dust were various in different coal plants. It was 250.8 mg/m3 averagely in Jiangxi patients of dust lung in coal separation plants of Jiangxi had not been found out, which suggested that the harmfulness in coal- cleaning plant was less than that in other positions of coal.   Conclusion   The powder dust concentration of some working positions in coal preparation plants were beyond the relative national standard, which would by potentially harmful to workers. Besides the management of coal preparation plants should be reinforced, powder dust-removing methods should be improved form eight aspects.
Effects of liver matrix or/and β-Mercaptoethanol on cultured cells from Schistosoma japonicum
QIN Jinhong, PENG Yan, DONG Huifen,
2005, 21(10): 1182-1183.
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  Objective   To study the effects of the liver matrix(bio-matrix from the liver of a rabbit)or/and β-M ercaptoethanol (β-Me)on the cultured cells from Schistosoma japonicum.   Methods   After schistosomula cells from 18-day old S. japonicum were prepared by a method of cold digestion, they were inoculated with the method of combination on general glass coverslips or the glass coverslips smeared with the liver matrix. The former was divided in to control andβ-Me groups, the later was liver matrix and combination(of β-Me and liver matrix)groups randomly. The cells in the control and liver matrix groups were cultured in the routine media, i.e., medium RPMI-1640 containing 20% calf serum and a moderate amount of antibiotics(penicillin G 100 iu/ml, streptomycin 100 μg/ml), while those in β-Me and combination groups were cultured in the routine media containing 50 μmol/L β-Me and l mmol/L sodium pyruvate. When cultured for 1, 2, 3 and 4 weeks, the cells were stained to show the activities of lactatedehydrogenase(LDH) by using the cytochemical method of enzyme, respectively. They were observed and photographed by the microscope(Olympus-BH2). The activities of LDH were measured by using the photomete-rimage analytical instrument(HPIA-2000)and analyzed statistically.   Results   The activities of LDH were the strongest in the cells from S. japonicumcultured for 1 week. Along with prolongation of the culture time, the activities of LDH became weaker gradually. In the first two weeks, the activities of LDH in the cells in β-Me group were similar to those in the combination group, and the group of the liver matrix similar to the control but weaker than the above groups. Quantitative analysis showed that the difference of LDH concentration in the cultured cells between β-Me, combination groups and the liver matrix, control groups was significant(P < 0.05). Three weeks later, the activities of LDH in the ceils of the liver matrix group were stronger than those in β-Me, combination and control groups, and the difference was significant(P < 0.05). However, no significant difference among the latter 3 groups were observe(P > 0.05).   Conclusion   The effects of the liver matrix on the activities of LDH in the cultured cells from S. japonicum were probably more effective and steady-going than those of β-Me. The enhanced effects of the liver matrix plus β-Me on the cultured cells was not observed in the study.
Study on association between gene polymorphism of Apolipoprotein E and attempted suicide
JIA Cunxian, ZHAO Zhongtang, GAO Lijie,
2005, 21(10): 1184-1185.
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  Objective   To understand the association between gene polymorphism of apolipoprotein E(Apo E)and attempted suicide.   Methods   220 paired suicide attempted and controls were analyzed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).   Results   There were four gene types of Apo E in each suicide attempters and controls group which were Apo E-E2/3, E3/3, E2/4 and E3/4 and in which Apo E-E3/3 was the main type. Multinomial model was used to analyze the factors which were related with attempted suicide with different Apo E gene type(attempters with Apo E-E3/4 being reference group), the results showed that affective conflict and dissatisfaction with life now were associated with attempters with Apo EE3/3, affective conflict were also associated with attempters with Apo E-E2/3, and no factors were associated with attempters with Apo E-E3/3. There were no association between different gene types of Apo E and attempted suicide by conditional logistic regression analysis.   Conclusion   This study didn't support the association between Apo E gene and attempted suicide. There were different factors related with suicide attempters with different gene type of Apo E.
Observation on safety and immune effect of live attenuated Hepatitis A vaccine(H2 strain) with suspension absorption technique in rhesus monkey
YANG Jingsi, CHEN Hongpo, YANG Wei,
2005, 21(10): 1186-1187.
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  Objective   To observe the safety and immune effect of live attenuated hepatitis A vaccine(H2 strain)produced by mono-layer absorption and suspension absorption with different virus harvest period in rhesus monkeys.   Methods   Fifteen rhesus monkeys with negative anti-HAV and normal level of alanine transaminase(ALT)were selected and divided randomly into 3 groups. A group of 5 animals for 1 specimen and 1ml of vaccine was injected by Ⅳ into each rhesus monkey. The local and systemic reactions were observed within 3 days after injection. Both anti-HAV and ALT were detected at wk 0, 2, 3, 4, 6, 8 and liver puncture was performed at wk 0, 4, 8 for histopathological examination. Autopsy was down at the end of observation.   Results   No local and systemic reactions were observed in any rhesus monkeys within 3 days after vaccination, The ALT levels were normal and no specific liver histopathological change were examined during whole period. The seroconversion rate of anti-HAV was 100%at 4 wk after inoculation. The GMTs were 9.2, 14.9, 27.9 for mono-layer absorption vaccine(26 d harvest), suspension absorption vaccine(23 d harvest) and suspension absorption vaccine(26 d harvest)at 4 wk and 32, 64, 64 at 8 wk. The immune effects induced by 2 suspension absorption vaccine were similar, but better than that mono-layer absorption.   Conclusion   Suspension absorption with 23 d harvest technique vaccine possesses good safety and immune effect in rhesus monkey inoculation. The application of suspension absorption(23 d harvest)technique for production of hepatitis A vaccine(H2 strain)is economic and practicable.
Significance of anti-HEV IgA detection in patients with acute HEV infection
CHEN Yan, TIAN Deying, XIA Ningshao
2005, 21(10): 1188-1190.
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  Objective   To establish enzyme-linked immunosorbent assays of anti-HEV IgA and to investigate its clinic significance in patients with hepatitis E.   Methods   An enzyme-linked immunosorbent assays of anti-HEV IgA was established by instead of anti-human IgG with anti-human IgA in anti-HEV IgG assays came from Beijing WanT ai biological pharmacy enterprise. Anti-HEV IgA, anti-HEV IgM and anti-HEV IgG were detected in serial sera of 60 patients with HEV-RNA positive and control groups.   Results   The 60 patients with HEV-RNA positive had both anti-HEV IgA and anti-HEV IgM alone. These patients were not only negative for HEV RNA but also lack anti-HEV IgG as well. Only one patient had both anti-HEV IgA and anti-HEV IgM (covert infection). Periodic serum samples obtained from 60 patients with hepatitis E were tested for HEV RNA, anti-HEV IgA, anti -HEV IgM and anti-HEV IgG. Their HEV-RNA were detectable in the serum until 1 to 40(20±11)d. But anti-HEV IgA were detectable until 1 to 150(120±23)d, anti-HEV IgM were detectable until 1 to 150(90±15)d. After two months of the onset the positive rates of anti-HEV IgA was higher than that of anti-HEV IgM(P < 0.05).   Conclusion   The method to detect the antiHEV IgA is practicable. Anti-HEV IgA is longer duration than that of anti-HEV IgM and as an assistant signal to diagnose acute hepatitis E without anti-HEV IgM. Anti-HEV IgA alone or along with anti-HEV IgM is useful for serological diagnosis of hepatitis E with increased specificity.
2005, 21(10): 1190-1190.
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Study on influence of Clonorchis sinensis/Clonorchiasis epidemic caused by ecological environment changes in weishan lake area
MIAO Feng, YAN Xianzheng, LIU Xin
2005, 21(10): 1191-1192.
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  Objective   To investigate the influence of clonorchis sinensis/clonorchiasis epidemic caused by ecological environment changes in Weishan lake area after the South-to-North water diversion project of China.   Methods   Epidemiological survey was performed on the natural environment. (Changes of water area and the infection of clonorchis sinensis/clonorchiasis buski in aquatic in weishan country)heath situation of the residents were investigated and the fresh stools from residents of lake areas were examined by improved kato-kato methods.   Results   The expansion of water area and control of water contamination after the South-to-North water diversion project were suitable for the propagation and spreading of the middle host of aquatic. The infective rate of Pseudorasbora parva infected by etacercarias of Clonor chis sinensis was 3.14%. The infective rate of the cats infected by adult of Clonorchis sinensis was 38.46%. But Clonorchis sinensis the sporocyst, redia and cercaria were not found in the freshwater Parafossarulus striatulus and Alocinma longicornis. Knowledge of Clonorchis sinensis/Clonorchiasis prevention and cure were short in the residents and the infection rate of fasciolopsis buski was 0.06%.   Conclusion   Changes of the ecological environment in weishan lake area and the unhealthy life style of the presidents may lead to the epidernic and spreading of Clonorchis sinensis/Clonorchiasis, but widely propagation of Clonorchis sinensis/Clonorchiasis prevention and cure knowledge and some methods can prevent the epidemic of Clonorchis sinensis/Clonorchiasis in the lake area.
Analysis on hepatitis C virus genotype and quasispecies in Yixing area
DING Weiliang, SHAO Jianwei, XU Ke,
2005, 21(10): 1193-1194.
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  Objective   To investigate the distribution of hepatitis C virus(HCV)genotypes and HCV quasispecies diversity in Yixing, Jiangsu province.   Methods   To identify the genotype of sera samples obtained from 163 donors who anti-HCV positive by the PCR with type specific primer, which were designed according to the sequence of 5cono-coding region(5'NCR). Genotypes distribution was investigated in patients with different sex, different ages and different kinds hepatitis C. To compare the HCV quasispecies diversity among the 43 patients with chronic hepatitis C.   Results   Of total 166 patients, 102 were HCV RNA positive in which 86 patients had genotype 1b(86/102;84.3%), 5 patients had genotype 2(6/102;5.9%), 5 patients was 1b/2 mixed types(5/102;4.9%)and genotype could not be determined in 5 patients. Distribution difference of HCV genotypes was found between female patients and male ones. The difference of HCV quasispecies diversity was found between the patients with normal or abnormal alanine transaminase(ALT).   Conclusion   Type 1b is predominant HCV genotype in Yixing area. Mix-infection rate is higher in females than that in males. Quasispecies diversity is corrected with ALT.
Inhibition mechanism of SIVmac239 on macques B lymphocyte(MM133)proliferation in vitro
YAO Zhenjiang, WANG Binyou, ZHAO Jingbo,
2005, 21(10): 1195-1196.
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  Objective   To explore the inhibition factors which CD4 +T lymphocyte secreted after infection with SIVmac239, whether or not these factors Could inhibit the Blymphocyte proliferation activity and it's inhibition mechanism, some helpful clue for pathogenology research of AIDS would be supplied.   Methods   Proliferation activity of MM133 cells treated by the supernatant of C8166 cells infected SIVmac239 tested by MTT assay. The inhibition of DNA synthesis of supernatant of C8166 cells infected by SIVmac239 on B cells estimated by 3H thymidine uptake assay. Whether or not the apoptosis happened when the DNA synthesis inhibiton judged by DNA fragmentation detection. Cyclin D1 expression was examined through western blot assay.   Results   SIVmac239 could stimulate CD4 +T lymphocyte to secret some inhibitant factors for the macques B lymphocyte. These factors could inhibit the DNA synthesis of B cells through 3H thymidine uptake assay, electrophoresis result did not show DNA ladder, there was not Cyclin D1 bands on western blot assay for the concentration of 25% and 12.5% supernantant of C8166 infected by SIVmac239.   Conclusion   CD4+T lymphocyte infected by SIVmac239 can produce some inhibitant factors on the macques B lymphocyte; these factors also can inhibit the DNA synthesis of B cells, but the factors do not induce the apoptosis of B cells. The major mechanism of inhibition of the factors on the B cells is to arrest the cells in G1 phase duing cell cycling. A new phenomenon has been found in this experiment, B cells can not be infected by SIVmac239, but they will be arrested in G1 phase by some factor produced by CD4 +T Lymphocyte infected with SIVmac239. Thus the function of B lymphocytes and the humoral immunity may be limited.
Study on protective cell immune mechanism of recombinant 31/32kDa antigens of Schistosoma japanicum
CHEN Xigui, SHEN Dingwen, LUO Jinping,
2005, 21(10): 1197-1198.
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  Objective   To study the effect of cell immunity in the protective immune mechanism of recombinant rSj31/32kDa antigens of Schistosoma japonicum(rSj31/32).   Methods   BALB/c mice were immunized three times with rSj31/32. ELISA kits were used to examine the levels of IFN-C and IL-4 before and 6 weeks after immunization. By the culture of spleen cells after challenge, IFN-C and IL-4 after stimulation with SEA were quantified by ELISA kits.   Results   The level of IFN-Cwere increased significantly a-f ter immunized with rSj31/32(P < 0.001). There was no obvious change in the level of IL-4. With spleen cells from rSj31/32 vaccinated mice, IFN-C was the most abundantly produced cytokine in response to SEA, while IL-4 secretion was the lowest(P < 0.01).   Conclusion   Cell immunity might play an important role in the protective immunity elicited by rSj31/32 antigen.
Toxic effect of Ascaris lumbricoides body fluid on intestinal epithelial cells
WENG Peilan, YUAN Keng, PENG Weidong,
2005, 21(10): 1199-1200.
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  Objective   To study the toxic effect of body fluid of Ascaris lumbricoides(ABF)on human intestinal epithelial cells (HCT-8 cells)and how the ABF concentration and inducing time could be related to the toxicity.   Methods   Survival rates, morphology and function of HCT-8 cells were compared in different ABF concentrations(i.e., 30, 100, 400 μg/ml)and different time in vitro.   Results   The observation from HE stain showed the most tox ic effect of HCT-8 cells at 800 μg/ml for 8 h. The toxicity of HCT-8 cels in each experiment group(25~800 μg/ml)was higher than that of matched control except 12.5% μg/ml.   Conclusion   The toxicity induced in vitro by ABF implies a dose-and time-dependent pattern in some extension. Meanwhile, ABF induced the apoptosis of HCT8 cells.
Effect of fucoidan on human leukaemia tumor cell line K562 apoptosis
WU Xiaomin, LUO Qiong, YAN Jun,
2005, 21(10): 1201-1203.
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  Objective   To study anti-tumor mechanism of fucoidan on human leukaemia tumor line K562.   Methods   The human leukaemia tumor cell lines were cultured in vitro. The effect of fucoidan to K562 was investigated by the single cell gel electrophoresis (SCGE), DNA ladder and FACS techniques after treated by water extract or ethanol extract from Laminaria japonica Aresch with doses of 0.5, 1, 2 and 3 mg/ml.   Results   Experimental study of MTT was used to determine the antitumor effects of fucoidan on inducing apoptosis of tumor cell, the dose of 1 mg/ml was remarkable. SCGE was used to detect the breakage of DNA strand and analyze damage of fucoidan to tumor cell. The result showed the damage was evident. The DNA ladder and FACS techniques showed the incidence rates of apoptosis were remarkably increased after treated 24 hours by fucoidan. The dose of 1 mg/ml increased significantly.   Conclusion   The anti-tumor effect of fucoidan was evident, and its mechanisms was probably involved the apoptosis of tumor cell which induced by fucoidan.
Preventive effect of Glycyrrhiza uralensis fisch on damage of genetic materials in male mouse reproductive cells
LIU Yang, QIAO Ping, ZHU Yuzhuo
2005, 21(10): 1203-1204.
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  Objective   To observe the preventive effect of Glycyrehize uralensis fisch on damage of genetic M aterials in male mouse reproductive cells induced by Pb(CH3MCOO)2.   Methods   Sperm abnormality test, Chromosome aberration of spermatogonia and unscheduled DNA synthesis(UDS)test in male mouse reproductive cells were used in the study.   Results   Glycyrrhiza uralensis fisch inhibited significantly sperm abnormality, chromosome aberration of spermatogonia an UDS in mouse reproductive cells induced by Pb (CH3COO)2(P < 0.01).   Conclusion   Glycyrrhiza uralensis fisch shows a protective effect, namely antimutagenesis, on injury of genetic materials in reproductive cells of male mouse.
Study on garlic oil combined resveratrol inducing apoptosis of human gastric carcinoma cell line MGC-803
ZHENG Guohua, LI Hao, LI Huiqing
2005, 21(10): 1205-1207.
Abstract(1439) HTML (315) PDF 295KB(73)
Abstract:
  Objective   To explore the effect of garlic oil combining with resveratrol inducing apoptosis of human gastric carcinoma cell line MGC-803.   Methods   To measure the inhibit effect of proliferation on garlic oil combining with resveratrol to gastric carcinoma cell by MTT. Apoptotic effect of gastric carcinoma cell by giemsa staining was observed. TUNEL assay, flow cytometry and confocal microscopy were used.   Results   The inhabiting rate of 42.8%, 59.8% and 65.5% could be measured after being exposed to garlic oil combining with resveratrol at the dosage 25μg/ml for 6h, 12h and 24h. Obvious cell apoptosis could be observed by giemsa staining and TUNNEL staining after exposed to combining medicine at 25 μg/ml for 24h. Positive cells of Annexin Ⅴ in group of garlic oil combined with resveratrol were higher than those of total positive cells of both garlic oil group and resveratrol group. The reverse results could be observed about DNA content within cells of the combined medicine group and the single medicine group by confocal microscopy.   Conclusion   The effect of apoptosis on garlic oil combined with resveratrol to MGC-803 cell line is obviously stronger than that of their single usage.
Study on association of HLA-DR antigen with esophageal carcinoma and Helicobacter pylori infection
MA Yanhua, WU Jing, YAN Xiang,
2005, 21(10): 1207-1208.
Abstract(1032) HTML (276) PDF 213KB(38)
Abstract:
  Objective   To investigate the relation between the HLA-DR expression and Helicobacter pylori infection in esophageal carcinoma.   Methods   HLA-DR was examined in 40 patients with esophageal carcinoma and 30 normal control subjects by immunohistochemistry technique(ABC)and relation between HLA-DR expression and clinicopathologic characteristics were analyzed. The status of H.pylori infection were determined by immunohistochemistry technique(S-P). All specimens were diagnosed through normal pathological process.   Results   Positive rate of HLA-DR in esophageal carcinoma was 45.0%, which was significantly higher than 13.3% in normal controls(χ2=7.977, P < 0.05), the expression of HLA-DR in intraepithelial carcinoma was significantly higher than that in invasive carcinoma(χ2=6.078, P < 0.05). The infection rate of Hp in esophageal carcinoma was 95%, which was significantly higher than 20% in normal controls. No significant associations were found between patients with HLA-DR antigen and Hp infection with regard to patients' age at presentation, Sex, tumor site, lymph node metastases, histological grading of tumor.   Conclusion   HLA-DR expression and Hp infection were associated with the incidence of esophageal carcinoma. The HLA-DR expression may prevent tumor invasion similarly to its role in intraepithelial carcinoma. The HLA-DR expression was not associated with the status of H.pylori infection.
Evaluation on organic teratogen of drinking water with micromass culture of rat embryo limb bud cells
WU Yuan, DING Rui, JI Yuan,
2005, 21(10): 1209-1211.
Abstract(984) HTML (223) PDF 365KB(22)
Abstract:
  Objective   To screen rapidly the teratogenic contaminants which were extracted from raw drinking water and tap water in Hefei city. An in vitro short-term test was carried out.   Methods   Organic contaminants were extracted from drinking water by use of XAD-4 macroreticular resins. With cultures of rat embryo limb bud cells for 5 days, the concentration inhibiting differentiation by 50% control(ID50)and the concentration inhibiting proliferation by 50% control(IP50)and the ratio of IP50/ID50 were gotten respectively by each inhibition curve.   Results   The extractives inhibited the differentiation and the proliferation of limb bud cells. The ID50 for tap water were 363. 1ml/ml(-S9)and 5.7 ml/ml(+S9), IP50 were 1355.2 ml/ml(-S9)and 1264.5 ml/ml(+S9), the ratios for IP50/ID50(-S9)were 3.7, IP50(+S9); IP50 were 223;The ID50 for raw water were 1.2ml/ml(-S9)and 0.387 ml/ml(+ S9); IP50 were 15.8 ml/ml(-S9)and 41.1 ml/ml(+S9), the ratios for IP50/ID50 were 13.5(-S9)and 106.2(+S\-9). That's to say, the extractives could be bioactivated into stronger inhibitors.   Conclusion   There parameters indicated the influence of organic contaminants on differentiation of rat limb bud cells was stronger than on proliferation, and those organics in drinking water belonged to a teratogenic agent and a specific inhibittors. The chlorination of drinking water should be studied more extently.
2005, 21(10): 1211-1211.
Abstract(871) HTML (238) PDF 129KB(60)
Abstract:
Effect of octachlorodipropylether on DNA damage of liver and lung cells in mice
TNA Weijun, WANG Xinru, XU Xikun
2005, 21(10): 1212-1214.
Abstract(819) HTML (208) PDF 275KB(46)
Abstract:
  Objective   To study the genetic toxic effect of octachlorodipropylether on mice liver and lung cells.   Methods   In an in vivo study, mice were sacrificed at 0, 3, 9, 24, 48 and 72 h after oral[0, 100, 200, 400 and 800 mg/(kgºbw)]administration of octachlorodipropylether. DNA damage was measured by the single cell gel electrophoresis(SCGE)assay.   Results   After oral administration[400 and 800 mg/(kgºbw)], DNA damage appeard in the liver at 9, 24 h, and in the lung at 9, 24 and 48 h.   Conclusion   A certain dose octachlorodipropylether has genetic toxic effect on mice.
2005, 21(10): 1214-1215.
Abstract(654) HTML (194) PDF 202KB(26)
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Study on DNA damage and proliferation of testicular cells with lead exposed
DONG Shuying, SHAN Yujuan, LU Mingjun
2005, 21(10): 1216-1218.
Abstract(787) HTML (259) PDF 365KB(26)
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  Objective   To study the toxicity mechanism of mice testicular cells by lead exposure.   Methods     Methods   of SCGE (Single cell gel electrophoresis technique)and MTT were used.   Results   There were different degree DNA damage of testicular cells by lead exposed to group(0.15%), group(0.3%), group(0.6%). The length of DNA migration were significant different to the control groups(P < 0.01). Lead restrained testicular cells proliferation. MDA level were increased and SOD and GSH activity were decreased.   Conclusion   Lead caused oxidative damage and DNA break damage of mice testicular cells, and restrained testicular cells proliferation.
2005, 21(10): 1218-1218.
Abstract(966) HTML (283) PDF 120KB(36)
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Changes of adrenomedullin level in nutrient obesity rats
YU Shangbin, LIU Shengyuan, LIU Lei,
2005, 21(10): 1219-1220.
Abstract(940) HTML (233) PDF 197KB(25)
Abstract:
  Objective   To observe the changes of adrenomedullin(ADM)level in plasma and heart/weight, liver/weight rate in nutrient obesity rats.   Methods   The concentration of ADM was determined by special radioimmunoassay, the weight of heart, liver and whole body were measured and the rate of heart/weight and liver/weight(g/g)were calculated respectively.   Results   The plasma ADM level in obesity rats(78.35±6.38)pg/ml was lower than that in normal rats(92.47±10.61)pg/ml(P < 0.05);the rate of heart/weight(g/g)and liver/weight(g/g)of obesity rats(0.319±0.019), (3.334±0.410)were higher than those in normal groups (0.225±0.020), (2.951±0.730)(P < 0.05).   Conclusion   Reduction of ADM level probably resulted in the occurrence and progression of nutrient obesity, and probably contributed to the increases of heart/weight and liver/weight.
Screening for anti-androgenic activities of acrylonitrile in viro
WANG Ning, ZHU Juyi, HUANG Jianshu,
2005, 21(10): 1221-1222.
Abstract(812) HTML (205) PDF 210KB(39)
Abstract:
  Objective   To investigate the anti-androgenic effect of acrylonitrile(AN)by the Hershberger assay in rats.   Methods   Acrylonitrile was administered orally at doses of 0, 10, 20, 30, 40 and 50 mg/(kgºbw)to castrated rats for 10 days. 0.4 mg/(kgºbw)testosterone propionate(TP)was also administered by subcutaneous injection after the administration of AN. In some rats, flutamide (FLU)was also administered orally at a dose of 25 mg/(kgºbw)as the reference chemical for(anti-)androgenicity. All rats were killed 24 h after the administration of the last dose. The sex accessory tissue weights, including the weights of prostate, seminal vesicle, penis, LA/BC muscle and Cowper's gland, and the weights of kidney and liver of rats were recorded.   Results   There were essentially no differences in sex accessory tissue weights between the negative control and any of the AN-treated groups(P > 0.05). The positive control flutamide[25 mg/(kgºbw)]showed a significant effect in this assay protocol(P < 0.05). There was no excessive systemic toxicity in this assay(P > 0.05).   Conclusion   AN mayn't have anti-androgenic properties on in vivo screening tests.
2005, 21(10): 1223-1224.
Abstract(827) HTML (195) PDF 194KB(22)
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2005, 21(10): 1224-1225.
Abstract(632) HTML (205) PDF 199KB(38)
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2005, 21(10): 1226-1227.
Abstract(778) HTML (223) PDF 311KB(40)
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2005, 21(10): 1228-1229.
Abstract(696) HTML (319) PDF 196KB(28)
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2005, 21(10): 1229-1229.
Abstract(778) HTML (208) PDF 127KB(26)
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Investigation and analysis of health system responsiveness of navy
LIU Xiaorong, CHEN Guoliang, WU Junsheng,
2005, 21(10): 1230-1232.
Abstract(1047) HTML (302) PDF 270KB(38)
Abstract:
  Objective   By investigating the health system responsiveness in some navy base, decisions and suggestions were made to maintain and advance the health of service men, meet the reasonable needs of service men, and to improve the health service.   Methods   Questionnaires were used in leaders, administrators of health organizations, health protectors, professional medical staff, patients and service men in division.   Results   70% percentage of the people considered responsiveness was very good or good. Total score of responsiveness was 7.839.   Conclusion   The general situation of health system responsiveness is good in some navy base, but problems still exist in troops below division. Health system responsiveness is a good method to evaluate the health system performance.
2005, 21(10): 1232-1232.
Abstract(670) HTML (185) PDF 135KB(29)
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Investigation on contamination by some species of Salmonella and Escherichia coli in retail meats in Shenyang
SUN Yan, TANG Xuemei, CONG Bailin,
2005, 21(10): 1233-1234.
Abstract(918) HTML (207) PDF 207KB(40)
Abstract:
  Objective   The objectives of this study were to determine the prevalence of Escherichia coli and Salmonella serovars in retail raw meats obtained from markets. A total of 500 samples of retail raw meat samples of chicken, duck, rabbit, pork, and beef were randomly obtained from ratail stores in 2 supermarkets, 6 farm markets, and 10 street markets in the city of Shenyang, from January 2003 to December 2003 and were examined for the presence of Escherichia coli and Salmonella serovars and the effects on microbial contamination with differences product type, season, and market. All chicken, duck and rabbit carcasses yielded E. coli contamination(100%). while 89% of beef chops and 85% of pork chops were positive for E. coli. About 61% of chicken, 71% of duck and 83% of rabbit carcasses were contaminated with Salmonella. About 40% of pork and 48% of beef chops were positive for Salmonella. Significant differences in the microbial prevalence were observed for supermarket-samples with the lowest contamination, street-market samples showing the highest contamination, and farm-market samples in the middle.
A surveillance and analysis on bone mineral density of 1 069 late pregnancy women in Zhoushan archipelage
CHEN Hailan, HUANG Fang, FU Meiqing,
2005, 21(10): 1235-1236.
Abstract(997) HTML (220) PDF 201KB(36)
Abstract:
  Objective   To learn the distribution discipline of the bone mineral density of the late pregnancy women and to provide the reference for guiding the calcium nutrition for the pregnancy women.   Methods   A quantitative ultrasound technoogy was develooped to measure the SOS of 1 069 late pregnancy women in Zhoushan archipelago, and the health status of the bone was evaluated through T-Score. The ANOVA analysis and the correlation and regression analysis procedues were conducted to fine out the changing discipline of the bone mineral density.   Results   391 women whose T-Score of SOS were less then-1.0, which was 36158 percent in total 1 069 late pregnancy women. The average values of SOS in age groups of 20~, 25~, 30~, 35~38 were respectively 4 079.39, 4 118.48, 4 154.05, 4 166.88(m/sec). There was a significantly statistical difference between that in the four age groups(F =23.73, P < 0.001). The best fitness model between the average value of SOS and age was Y=3 237162+264152ln(X)(r=01 91, F=77.97, P < 0.01). The best fintnes smodel between the average value of SOS and BMI was Y=-0.52X2+23183X+3 855.66(r=0.89, F=12.72, P=0.004 7).   Conclusion   The mass of bone mineral decreased in 36.58 percent pregnancy women and that reached the criterion of osteoporosis in 1.40 percent pregnancy women. The pregnancy women lost some bone mineral during the pregnancy period. However, the bone mineral density increased prograssively from 20 to 38 years old. There is a relationship of positive correlation between the BMI and loss of bone mineral.
2005, 21(10): 1237-1237.
Abstract(771) HTML (184) PDF 133KB(26)
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2005, 21(10): 1238-1239.
Abstract(596) HTML (177) PDF 188KB(28)
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2005, 21(10): 1239-1239.
Abstract(705) HTML (234) PDF 118KB(32)
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2005, 21(10): 1240-1241.
Abstract(670) HTML (173) PDF 192KB(25)
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2005, 21(10): 1243-1244.
Abstract(650) HTML (164) PDF 205KB(31)
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Study on determination of taurine and its stability in beer by fluorophotometry
LIU Hui, XU Guohui, FENG Fumin,
2005, 21(10): 1244-1245.
Abstract(824) HTML (207) PDF 208KB(60)
Abstract:
  Objective   To determine the content of taurine in beer by fluorophotometry and its stability in beer.   Methods   The normal beer was incorporated with taurine at five levels and kept under 4℃ and normal temperature respectively for use. Freshly incorporate beer was made six months later and was determined with the formerly incorporate beers all together. After pretreatment, the sample was separated and cleaned up with cation ex change column, then taurine was derived by o-phthalaldehyde and was monitored by fluorophotometry.   Results   T he fluorophotometry for taurine analysis in beer showed the precision(relative standard deviation)was less than 2.4%, the recoveries were more than 95%and the detection limit was 0.023 mg/L; The differences of taurine content among all the samples were not statistically significant(P > 0.05).   Conclusion   The method is rapid, simple, with a good reproducibility and is suitable of analysis of taurine in beer; the good stability of taurine in beer ensures the feasibility of taurine-contained hygienical beer.
2005, 21(10): 1246-1246.
Abstract(761) HTML (198) PDF 128KB(22)
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2005, 21(10): 1247-1247.
Abstract(795) HTML (241) PDF 125KB(30)
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2005, 21(10): 1248-1248.
Abstract(646) HTML (186) PDF 118KB(35)
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2005, 21(10): 1249-1249.
Abstract(520) HTML (128) PDF 118KB(21)
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2005, 21(10): 1250-1250.
Abstract(508) HTML (206) PDF 124KB(36)
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2005, 21(10): 1251-1252.
Abstract(626) HTML (209) PDF 113KB(28)
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2005, 21(10): 1253-1254.
Abstract(830) HTML (204) PDF 196KB(24)
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2005, 21(10): 1254-1255.
Abstract(618) HTML (141) PDF 185KB(19)
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2005, 21(10): 1256-1258.
Abstract(689) HTML (180) PDF 243KB(34)
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2005, 21(10): 1258-1258.
Abstract(741) HTML (244) PDF 129KB(45)
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2005, 21(10): 1259-1259.
Abstract(637) HTML (135) PDF 143KB(42)
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2005, 21(10): 1260-1262.
Abstract(709) HTML (242) PDF 263KB(29)
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2005, 21(10): 1262-1262.
Abstract(647) HTML (198) PDF 132KB(27)
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2005, 21(10): 1263-1264.
Abstract(818) HTML (240) PDF 204KB(36)
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2005, 21(10): 1265-1267.
Abstract(838) HTML (297) PDF 267KB(56)
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2005, 21(10): 1267-1267.
Abstract(777) HTML (205) PDF 124KB(39)
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2005, 21(10): 1268-1269.
Abstract(773) HTML (225) PDF 319KB(27)
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Investigation on ealth status and service utilization among community inhabitants in Nanjing
ZHANG Kaijin, ZHOU Ling, TANG Bocai,
2005, 21(10): 1270-1271.
Abstract(1225) HTML (246) PDF 207KB(79)
Abstract:
  Objective   To understand health status of the community inhabitants and to take community diagnosis.   Methods   12 878 community inhabitants were surveyed in Nanjing.   Results   The total prevalence rate of the chronic diseases was 26.6%.The prevalence rate of latent hypertension was 14.8%.The control rate of blood pressure was 35.4% among hypertension patients.The disease prevalence rate in the past two weeks was 30.8%.The rate of seeking medical advice in the past two weeks was 13.0%.The rate of being in hospital in the past one year was 6.9%.   Conclusion   The community health care must be developed.Prevention of the chronic diseases should be taken in the community.
Effect of nutritional education on improvement of knowledge, attitude and practice with calcium nutrition in pupil
LIU Pei, WANG Canlan, ZHAO Huashuo,
2005, 21(10): 1272-1273.
Abstract(979) HTML (194) PDF 209KB(24)
Abstract:
  Objective   To investigate the knowledge, attitude and practice with calciumnutrition in pupil and to explore the effective ways of nutritional education.   Methods   Clusterrandomization sampling was adopt in 1 477 pupils and their parents in 4 primary schools inXuzhou city to compare changes in the knowledge, attitude and practice with calcium nutrition.   Results   Pupils were easy to accept the nutritional education and their levels of knowledge withcalcium nutrition and diet practice were significantly improvement.   Conclusion   Pupils' calciumnutrition situation can be improved by effective nutritional education.
Relation between environmental pollution caused by free SiO2 dust and lipid peroxidation in erythrocyte membrane in human
HU Dalin, LIU Yiming, TANG Dongsheng,
2005, 21(10): 1273-1274.
Abstract(804) HTML (196) PDF 210KB(24)
Abstract:
  Objective   To explore the relation between air environmental pollution by free SiO2 dust and lipid peroxidation degree in erythrocyte embrane in human.   Methods   179 healthy workers exposed to free SiO2 dust and 57 healthy members(control group)were sampled by cluster sampling method in two ceramics factories in Foshan city of Guangdong province, and a cross sectional investigation was carried out in up-related population and 2 ml blood in vein was draw from each one. Malondialdehyde(MDA), superoxide ismufase(SOD)and glutathion peroxidase(GSH-px)were tested by method introduced in reagent kits.   Results   Activity of GSH-px in erythrocyte membrane in workers exposed to free SiO2 dust was significantly lower than that of control group(P < 0.05), the quantity of MDA in erythrocyte membrane in workers was significantly higher than that of control group(P < 0.05), and activity of SOD in workers was significantly lower than that of control group(P < 0105).   Conclusion   Air environmental pollution by free SiO2 dust can cause significant toxicological reaction of lipid peroxidation in erythrocyte membrane in human, the change of degree of lipid perox idation maybe a certain kind of pre-clinical message, and sustain a stable balance state of anti-oxidation system maybe an important strategy for the secondary prevention of toxicological reaction caused by free SiO2 dust in human.
2005, 21(10): 1275-1276.
Abstract(861) HTML (248) PDF 198KB(39)
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2005, 21(10): 1276-1277.
Abstract(572) HTML (230) PDF 253KB(24)
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2005, 21(10): 1278-1280.
Abstract(745) HTML (209) PDF 248KB(29)
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2005, 21(10): 1280-1280.
Abstract(667) HTML (191) PDF 123KB(25)
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